The effect of swimming training on the oppoptosis in lung tissue of type-1 diabetic mice

Abstract

To date, beneficial effects of swimming training have been shown in different inflammatory condition such as diabetes. However, the precise mechanism by which swimming training exerts its therapeutic effect has not yet been determined. Therefore, the current study was conducted to investigate the effects of swimming training on apoptosis -related genes in lung tissue of type -1 diabetic mice. Methods: In this study, thirty-two male mice were allocated into four groups; Control group (C), Swimming group (Swimming), Diabetic group (D) and Diabetic + EX (D+ EX) group. In diabetic groups, for induction of diabetes, a single dose of streptozotocin (50 mg / kg) was injected intraperitoannally. Two weeks ofter induction of diabetes, swimming training was performed three times per week for four weeks. Forty-eight hours after the last swimming training, the animals were sacrificed and their lung tissue was obtained. Pathological injuries were assessed using H&E staining. The expression of IL-1β and apoptosis -related genes was measured using real-time PCR assay. Results: Histological examination revealed the promotion of pathological changes in lung tissue of diabetic groups coincided with the up-regulation of IL-1β, Caspase-3 and Bax and up-regulation of Bcl-2 compared to the C group. Swimming training improved diabetic-induced changes.