Preparation of self-nano emulsifying drug delivery system (SNEDDs) of buprenorphine and evaluation of its analgesic effect on mice

Abstract

Introduction: Increased absorption and lymphatic transport of drugs after loading in lipid drug delivery systems such as self-emulsifying systems can also increase oral bioavailability. Objective: In this project, the analgesic effect of Self-Nanoemolsifying Drug Delivery Systems containing the drug buprenorphine was investigated in male albino mice.Methods: In this study, the best SNEDDS formulation with the best solubility was selected and loaded with buprenorphine. 7 groups of 10 male albino mice weighing 20 to 25 grams were included in this study. The grouping was as follows: 1) SNEDDS formulation without buprenorphine , 2) 0.25 mg/kg formulation, 3) 0.5 mg/kg formulation, 4) 1 mg/kg formulation, 5) 0.25 mg/kg of pure drug powder, 6) 0.5 mg/kg of pure drug powder, 7) 1 mg/kg of pure drug powder. The analgesic effect of the drugs was investigated using the hot plate test with a temperature of 54 degrees. Data were analyzed using GraphPad software and One Way ANOVA test.Results: The findings showed that the formulation prepared in all time intervals and in all doses had a longer tolerance period in the hot plate test than the drug powder (p < 0.001). The amount of tolerance of mice in the hot plate test in the second group increased with the passage of time from 1 to 3 hours, and in the case of groups 3 and 4, it decreased significantly (p < 0.05). Also, the average percentage of mpe in all doses in the prepared formulation was significantly higher than the powder (p < 0.001). Conclusion: Based on the findings of this study, the nanoemolsifiable formulation of buprenorphine has better analgesic effects than the powder of the drug itself in doses of 0.25, 0.5, and 1 mg/kg, as well as time intervals of 0.5, 1 and It was 3 hours.