School of Traditional Medicine

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Subject: search.filters.subject.Apoptosis

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    Anti-cancer Effect of Mummy on Breast Cancer Cell Lines (MCF7)
    (Tabriz University of Medical Sciences, School of Traditional Medicine, 2020) rahmani barouji, solmaz; fazljou, seyyed mohammad bagher; Traditional Medicine; Yari Khosroushahi, ahmad; Torbati, Mohammadali
    Background: Mummy (Iranian pure shilajit) is a remedy with possessing anti-inflammatory, antioxidant and anticancer activities. This study aimed to examine mummy effects on epithelial-mesenchymal transition (EMT) and invasiveness of MCF-7 and MDA-MB-231 breast cancer (BC) cell lines with underlying its mechanism. Materials and Methods: The dose-dependent inhibitory effect of the mummy on cell proliferation in vitro was determined using the MTT assay. Flow cytometry and 4',6-diamidino-2-phenylindole dihydrochloride staining were respectively used for quantitative and qualitative analysis of cellular apoptosis, and gene expression analysis was conducted using real-time PCR. Results: MDA-MB-231 showed more sensitivity than the MCF-7 cell line to the anticancer activity of mummy, while mummy did not exhibit significant cell cytotoxicity against human normal cells (MCF‐10A). The gene expression profile demonstrated a significant decrease in TGF-β1, TGF-βR1, TWIST1, NOTCH1, CTNNB1, SRC along with an increase in E-cadherin mRNA levels in mummy treated cells compared to the untreated control group (p ≤0.05). Conclusions: Mummy triggers inhibition of EMT and metastasis in breast cancer cells mainly through the downregulation of TGFβ1 activity, and more studies required to find its specific anticancer activity with details.
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    The effect of sericin on anxiety, depressive-like behaviors, oxidative stress as well as apoptosis induced by chronic restraint stress in male mice
    (Tabriz University of Medical Sciences, School of Traditional Medicine, 2019) Banagozar Mohammadi, Ahad; Sadigh-Eteghad, Saeed; Fazljou, Seyyed Mohammad Bagher; E.J. Golzari, Samad; Traditional Medicine; Mahmoudi, Javad; Torbati, Mohammadali
    Introduction: Stress, depression and anxiety are now considered as the most prevalent disease in the century. Long-term exposure to stress through different mechanisms causes complications and consequences. Prevention of destructive effects of stress and providing appropriate preventive and therapeutic methods are of illicit drug importance. Objective: Since years away in traditional Iranian medicine, the therapeutic uses of silk are especially common for the treatment of depression, memory and brain function enhancement. Considering the traditional medicine literatures on the insure of silk effect in a wide range of brain diseases, in this study, the protective effects of sericin as the main protein of silk is investigated in prevention of stress-induced brain complications. Methods: Animals were subjected to chronic inhibitory stress for 3 hours and within 21 days sericin were administrated at doses of 100, 150 and 200 mg/kg/day. At the end of the period, behavioral tests were used to evaluate depression and anxiety. Then, blood samples were obtained to measure serum corticosterone level and prefrontal cortical as well as hippocampal samples were used to evaluate the level of oxidative stress and apoptosis markers. Results: Sericin increased percentage of presence in the central area of the Open Field Test, percentage of frequencies and spent time in open arms of Elevated Plus Maze. Also it decreased the immobilization time in the Tail Suspension and Forced Swimming Tests. Sericin administration decreased lipid peroxidation levels and increased total antioxidant capacity (TAC) and GPx and SOD activities in the prefrontal cortex and hippocampus. Sericin modulates serum corticosterone concentration and inhibits apoptosis by decreasing Bax to Bcl-2 ratio and decreasing Caspase 3 levels in these regions. Results: The findings indicate the protective effect of sericin in the face of chronic restraint stress and prevention of behavioral, psycho-pathological and molecular changes.