School of Traditional Medicine

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Anti-cancer Effect of Mummy on Breast Cancer Cell Lines (MCF7)
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2020) rahmani barouji, solmaz; fazljou, seyyed mohammad bagher; Traditional Medicine; Yari Khosroushahi, ahmad; Torbati, Mohammadali
Background: Mummy (Iranian pure shilajit) is a remedy with possessing anti-inflammatory, antioxidant and anticancer activities. This study aimed to examine mummy effects on epithelial-mesenchymal transition (EMT) and invasiveness of MCF-7 and MDA-MB-231 breast cancer (BC) cell lines with underlying its mechanism. Materials and Methods: The dose-dependent inhibitory effect of the mummy on cell proliferation in vitro was determined using the MTT assay. Flow cytometry and 4',6-diamidino-2-phenylindole dihydrochloride staining were respectively used for quantitative and qualitative analysis of cellular apoptosis, and gene expression analysis was conducted using real-time PCR. Results: MDA-MB-231 showed more sensitivity than the MCF-7 cell line to the anticancer activity of mummy, while mummy did not exhibit significant cell cytotoxicity against human normal cells (MCF‐10A). The gene expression profile demonstrated a significant decrease in TGF-β1, TGF-βR1, TWIST1, NOTCH1, CTNNB1, SRC along with an increase in E-cadherin mRNA levels in mummy treated cells compared to the untreated control group (p ≤0.05). Conclusions: Mummy triggers inhibition of EMT and metastasis in breast cancer cells mainly through the downregulation of TGFβ1 activity, and more studies required to find its specific anticancer activity with details.
Assessment of effect of jadwar capsule (Delphinium Denudatum) on clinical manifestation of knee Osteoartheritis versus pelacebo : A double blind randomized controlled clinical trial
(Tabriz University of Medical Sciences, School of traditional medicine, 2022) baghervand navid, roghayeh; ghojazadeh, morteza; tradtinal medicine; karimi, mehrdad; fazljou, seyed mohammad bagher
Abstract Introduction: Osteoarthritis is the most common arthritis which is characterized by degeneration of articular cartilage. The most important symptoms are joint pain, stiffness, movement, deformity, different effect from the subject. Osteoarthritis is rated as the fifth cause of the year of life last index in high income countries and ninth in low to middle income countries. Due to complications of medical and surgical interventions and the growing demand to herbal medicine, the research on the medicinal plants can be decide as a beneficial strategy. One of the most useful plants in the Traditional Persian Medicine is Jadwar (Delphinium denudatum wall.).Jadwar is used as medicinal plant in many diseases in Traditional Persian Medicine. One of the most important usages of Jadwar in Traditional Persian Medicine is for joint problems like, arthralgia, joint stiffness, and arthritis. Aim: Determining the effect of Jadwar (Delphinium denudatum wall.) on clinical manifestation of knee Osteoarthritis versus placebo Methods: 104 patients with mild to moderate knee osteoarthritis met the inclusion criteria and after obtaining informed consent, detailed history and clinical examination were performed. Patients allocated to the two equivalent groups of 52 people (intervention and control groups) randomly using the block design method. Jedwar and placebo capsules were identical in appearance and were placed in the same shaped containers, and in order to the allocation concealment, by someone other than the researchers, the drug containers were placed in identically numbered seeled envelopes. In the next step the intended drug (jadwar and placebo capsules) prescribed to patients. Patient consumed the drugs two times a day for four week. The outcomes, including pain, stiffness, and physical activity were evaluated using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) questionnaire and the Visual Analogue Scale (VAS) at baseline two and four weeks after the intervention. Results: The statistical comparison between intervention and control groups showed that the jadwar significantly reduces the pain compared with control group according to the vas questionnaire (P=0.0000). Also according to the womac questionnaire the statistical analysis showed that the jadwar reduces the pain and stiffness of knee and improves the physical activity of patients better than placebo. This effect was statistically significant at the end of the second week for VAS index and improvement of physical activity by WOMAC index and for all indices in the fourth week (end of study) (P = 0.000). Conclusion: This study showed that taking oral capsules twice a day orally on the symptoms of patients with mild to moderate osteoarthritis for one month has a better effect on reducing pain, dryness and improving patients' daily activities than placebo capsules.
Assessment of effect of topical palm date (phoenix dactylifera L.) preparation on clinical manifestation of knee Osteoarthritis versus Diclofenac gel : A randomized clinical trial
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2020) Bagherzadeh-karimi, Alireza; Fazljou, Mohammad bagher; Zargaran, Arman; Traditional Medicine; Elmi, Asghar; Karimi, Mehrdad
Abstract Introduction: Osteoarthritis is the most common arthritis. Osteoarthritis is rated as the fifth cause of the year of life last index in high income countries and ninth in low to middle income countries. Due to complications of medical and surgical interventions and the growing demand to herbal medicine, the research on the medicinal plants can be decide as a beneficial strategy. One of the most useful plants in the Traditional Persian Medicine is date palm (Phoenix Dactylifera L.).Date palm (Phoenix Dactylifera L.) is used as medicinal plant in many diseases in Traditional Persian Medicine. One of the most important usages of date palm in Traditional Persian Medicine is the topical use of date fruit for joint problems like, arthralgia, joint stiffness, and arthritis. Aim: Determining the effect of topical palm date (phoenix dactylifera L.) preparation on clinical manifestation of knee Osteoarthritis versus diclofenac gel: A randomized clinical trial Methods: According to the inclusion criteria, ninety four patients with mild to moderate knee osteoarthritis were included in the study. Informed consent was obtained from patients then precise history was obtained from patients and clinical exams were done. Patients allocated to the two equivalent groups of 47 people (intervention and control groups) randomly using the block design method. In the next step the intended drug (date pomade and diclofenac gel) prescribed to patients. Patient consumed the drugs three times a day and one fingertip index for four week. The vas and the womac questionnaires were collected before the starting the medication and week 2 and 4 after intervention by interviewing. Results: The statistical comparison between intervention and control groups showed that the date's pomade significantly reduces the pain compared with control group according to the vas questionnaire (P=0.0000). Also according to the womac questionnaire the statistical analysis showed that the date pomade reduces the pain and stiffness of knee and improves the daily physical activity of patients better than diclofenac gel. But this reduction of pain and stiffness and improvement of the physical activity was significant at the end of week 4 after the intervention (P=0.0000) and was no significant at the end of week 2 after the intervention (P=0.90, P=0.05, P=0.05 for pain, stiffness, and physical activity respectively). Conclusion: This study showed that the consumption of date pomade three times a day can reduce the pain and stiffness of the mild to moderate osteoarthritis patient's knee and improve the daily physical activities of those patients better than diclofenac gel.
Assessment of the Effect of Melissa Officinalis L. Hydro Alcoholic Extract on Anxiety and Depressive-Like Behaviors in a Mice Model of Chronic Restraint Stress
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2021) Ghazizadeh, javid; Hamedeyazdan, Sanaz; Torbati, Mohammadali; Fakhari, Ali; Traditional Persian Medicine; Araj-khodaei, Mostafa; Sadigh-Eteghad, Saeed
Background, the prevalence of mood disorders is increasing gradually. At the same time, physicians are increasingly interested in using herbal medicines based on patients' beliefs. Since lemon balm (Melissa officinalis L.) is one of the famous medicines in traditional Iranian medicine for the treatment of mood disorders, it led us to evaluate the effects of lemon balm on depression and anxiety. Aim, this study evaluated the effects of a hydro-alcoholic extract of Melissa officinalis (HAEMO) on anxiety- and depressive-like behaviors, oxidative stress and apoptosis markers in restraint stress-exposed mice. Methods: First, we prepared the hydro-alcoholic extract of lemon balm in vitro and then assayed the amounts of phytochemical compounds of total phenols, flavonoids, antioxidant activity and the active ingredient of rosmarinic acid in the extract. in order to induce a depression-like model, mice were subjected to restraint stress (3 h/day) and received normal saline or HAEMO (50, 75 and 150 mg/kg/day) for 14 days. The behavioral changes in an open field task, elevated plus maze, tail suspension and forced swimming tests were evaluated. Also, malondialdehyde (MDA) levels and enzyme activities of superoxide dismutase and glutathione peroxidase, and total antioxidant capacity were assessed in the prefrontal cortex and hippocampus. Moreover, levels of Bcl-2, Bax and caspase 3 in the brain as well as serum concentration of corticosterone were evaluated. Results: HAEMO (75 and 150 mg/kg) significantly reversed anxiety- and depressive-like behaviors. Also, HAEMO reduced MDA levels, enhanced enzymatic antioxidant activities and restored serum levels of corticosterone. An immunoblotting analysis also demonstrated that HAEMO decreased levels of pro-apoptotic markers and increased anti-apoptotic protein levels in the prefrontal cortex and hippocampus of restraint stress-exposed mice. Conclusion: Our findings suggested that HAEMO reduced inflammation and had anxiolytic and anti-depressant effects in mice.
Celery plus Anise Versus Metformin for the Treatment of Oligomenorrhea in the Polycystic Ovary Syndrome: A Triple-blind Randomized Clinical Trial
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2018) Moini Jazani, Arezoo; Tansaz, Mojgan; Nazemyieh, Hossein; Sadeghi Bazargani, Homayoun; Traditional medicine; Hamdi, Kobra; Fazljoo, Seyed Mohammad Bagher
Background: Polycystic ovary syndrome (PCOS)-induced oligomenorrhea can leave psychological and non-psychological impacts on women. Among the proposed strategies for its treatment, herbal medications are of importance due to favorable effect profiles. Objectives: We planned a study to compare the effects of Celery and Anise combination (CAC) with metformin (met.) on oligomenorrhea in PCOS patients. Methods: We conducted a triple-blind clinical trial, and 72 patients were randomly allocated into two equal groups and received six capsules of either CAC (750 mg each) or met. (250 mg each) in 3 divided doses for 15 days beginning with the follicular phase. If the bleeding occurred, three capsules of either placebo or met. would be administered daily during menstruation phase, and then the follicular phase step with six capsules would be repeated. If the bleeding did not occur, three capsules of either placebo or met. would be administered each day for 15 days. The regularity of menstrual bleeding as the primary outcome, as well as testosterone, luteinizing hormone/follicular stimulating hormone ratio (LH/FSH), and complications were assessed before and after three cycles. Results: The mean age ± standard deviation of all patients was 26.5±6.1. Also, mean Body Mass Index was 26.4±3.5. CAC significantly improved oligomenorrhea (58.3% vs 25%, p<0.01), increased bleeding episodes (p=0.003) and reduced testosterone (mean difference 0.16 vs -0.02, p=0.005) and LH/FSH (mean difference 0.75 vs -0.08, p=0.002) without any major side effects compared to the met. group. Conclusions: we showed that CAC can regulate menstrual cycles and improve oligomenorrhea in PCOS patients superiorly to met.
Co-delivery of methotrexate and extracted berberine from berberine vulgaris-Lby PAMAM dendrimer for evaluation of their anticancer effect on HeLa cancer cell line compared to each one berberin and methotrexate effect alone invitro
(Tabriz University of Medical Science -School of Traditional Medicine, 2021) Majidzadeh, Hossein; Torbati, Mohammadali; Traditional Medicine; Ezzati Nazhad Dolatabadi, Jafar; Araj-Khodaei, Mostafa
Background and objective: Cervical cancer is the second most common cancer after breast cancer among women throughout the world. Conventional strategies treatments include chemotherapy, surgery and radiotherapy. Chemotherapy is more selective to treat cervical cancer but unfavorable side effects limited it applications. Combination treatment and a variety of drug delivery systems including nanoparticles are used to reduce the complications of chemotherapy. Materials and Methods: The formation of MTX conjugated PAMAM transform loaded with BER (MTX-PAMAM-BER) had been evaluated by (FT-IR), and particle size evaluated through (DLS). In regarding to biological assay, MTT assay was carried out to estimate the cytotoxicity of one and combined groups, followed by apoptosis assay to calculate the number of apoptotic cells. Both DAPI staining and DNA ladder were complemented tests to illuminate morphological changes and DNA fracturing on HeLa cancer cells. Results: The characterization of formulated nanostructure showed that the conjugation of MTX and loading and BER into PAMAM dendrimer was carried of properly. The obtained results from MTT, flow cytometry, fluorescence microscopy and DNA fragmentation assays revealed that presence of MTX on PAMAM nanoparticles, particularly the co-delivey system (PAMAM-MTX-BER) had higher cell cytotoxicity, apoptosis and DNA fragmentation against HeLa cells over 48 hour. Conclusio: BER mixed with MTX can be considered as an effective therapy to reduce growth and induce apoptosis in HeLa cancer cells. Also, surface modification of PAMAM nanoparticle with MTX and BER make it a suitable platform for active delivery drugs into cancer cells and had higher drug transfection efficiency.
Comparison of medicaleducation in Rab-e- Rashidi university of Tabriz with medical education in medical sciences University of Tabriz
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2020) Jabbarzadeh Esfanjani, Amirollah; Khalili, majid; History of Medical Sciences; Jabbari Beyrami, Hossein
Introduction: Providing capable and efficient human resources is the main task of the university that requires the use of appropriate models, especially historical and national experiences. The purpose of this study was to compare the methods of medical education at Rab-e- Rashidi University of Tabriz in eighth century AH and compare it with medical education at Tabriz University of Medical Sciences as one of the leading universities in the country to introduce applicable issues. Methods: In this mix method study, measurable indicators of medical educations were extracted from related litterateurs and finalized by Delphi technique. In the following the Expert-approved indicators in two universities were extracted, compared and Recommended for use. Finding: The findings of the Indicator Section include 27 articles that are displayed in the Summary Table. From the above articles, 30 indices were extracted and 20 indices were approved by experts for comparing the two universities. The comparative table covers the areas of stewardship, education, research, and services. Discussion and conclusion: Rabee Rashidi University has made significant progress in its time which a number of them are applicable at present situations of Iran medical universities. As a result, we recommend at least thirty practical tips for implementation.
Comparison of the effect of Citrus Aurantium Flower Hydroalcoholic Extract with Mefenamic Acid on Severity of Primary Dysmenorrhea
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2020) Aboualsoltani, Farkhondeh; Fazljou, Seyyed Mohammad Bagher; Traditional Medicine; Bastani, Parvin; Khodaie, Laleh
Introduction: Primary dysmenorrhea is one of the most common complaints of women with adverse effects on social, economical and psychological states of their life. Medicinal plants, which have been reported beneficial in treatment of different aspects of this problem in Persian traditional medicine, can be a suitable method for treating disorder. Our aim was to study the effect of Citrus aurantium(C.A) flower extract on Primary dysmenorrhea, bleeding time and possible side effects. Methods: This study was a randomized double blind clinical trial, in which with convenient sampling, 105 Primary dismenorrhea patients aged 15 to 20 years were randomly(with RandList software) assigned to three groups of Citrus aurantium flower capsules, mefenamic acid capsules, or placebo capsules (35 participants in each). Depending on their group, they consumed 250 mg capsule of Mefenamic acid, Citrus aurantium flower or Placebo 3 times a day for first 3 days of two consecutive cycles. They marked the severity of the pain from 0 to 10 on McGill pain ruler. Data was analized by SPSS22. Results: The results of repeated measures analysis of variance, showed that the severity of dysmenorrhea and days of bleeding in C.A flower and mefenamic acid groups were significantly lower than before the intervention and compared to the placebo group (P=0.000). The reduction in severity of pain in the C.A flower group was also significantly higher than that of the mefenamic acid group (P= 0.000). C.A flower group reported the least side effects(82.4% without reported side effects). Also significant reduction of bleeding time was observed in the intervention groups (P=0.000). Conclusion: Due to higher effect of Citrus aurantium flower in reducing the severity of primary dysmenorrhea compared to Mefenamic acid, very low side effects, high satisfaction with its consumption and ease of access, this herb can be a good alternative for treating patients with primary dysmenorrhea.
Comparison of the effectiveness of pistacia lentiscus oil and sesame oil on inflammatory and histopathological factors of ulcerative colitis in animal models (rat)
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2020) maryam, ostovan; Torbati, Mohammad ALI; Traditional Medicine; Khazraei, Hajar; Fazljou, Seyyed Mohammad Bagher
Introduction: Pistacia lentiscus belongs to the Anacardiaceae family. Its resin has anti-inflammatory and antioxidant properties. The aim of this study was to evaluate the effects of mastic on cytokines TNFα،interleukin 6 and myeloperoxidase in rats with colitis. Method & Material: 48 male Sprague-Dawley rats were randomly divided into six groups: the first group of rat without colitis induction and by injection of normal saline through the anus, the second group of rat without colitis induction without treatment and only with saline injection through the anus. The third group of rat with induction of colitis and prednisolone administration (5 mg per kg per day); and the fourth group of rat with induction of colitis and administration of mastic oil (400 mg / kg / day) orally, the fifth group of rat with administration of mastic oil intra-rectal. The sixth group of rat induced colitis with sesame oil orally for six consecutive days. Effects of treatment were assessed by determining the cytokines interleukin 6 and TNFα and myeloperoxidase (MPO) activity, macroscopic scores and histopathological parameters. The results of each group were compared with rat in the colitis group by administering normal saline in the form of enema (rat of the second group). Results: After consuming sesame oil orally, the amount of myeloperoxidase in comparison with colitis was significantly reduced by administration of normal saline (P=0/025) rectally. Administration of mastic oil (400 mg / kg orally) in reducing the severity of colitis by reducing Total colitis index (p=0/046) was effective after six days. Rectally administration of mastic significantly reduced TNFα similar to the prednisolone orally compared to untreated colitis (p=0/024). Interleukin 6 did not change in the mastic orally and rectally and sesame oil oral groups. Conclusion: According to our results, mastic oil and sesame oil reduce inflammatory cytokines and this indicates that they can be used as natural resources to reduce the inflammation of ulcerative colitis.
Comparison of traditional medicines of Rheum Palmatum versus Paeonia Officinalis and Mefenamic acid on primary dysmenorrhea: a randomized clinical trail
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2018) Rajabzadeh, Farrin; Khodae, Laleh; Sahebi, Leyla; Traditional medicine; Fazljoo, Seyed Mohammad Bagher; Abbasalizadeh, Shamsi
More than half of the society is women. Primary dysmenorrhea is one of the most common abnormalities in women that has been cited various traditional therapies in Persian medicine for this disorder. We aimed to assess and compare the effects of Rheum Palmatum (RP), Paeonia Officinalis (PO) and mefenamic acid on primary dysmenorrhea. In this double – blind randomized clinical trial study 105 students residing in the dormitory of Tabriz University of Medical Sciences from 18 to 35 years old were randomly divided into three groups. During the first cycle, the control cycle (placebo) was given to the patients and patients were asked to respond to the degree of severity and duration of pain along with other symptoms. During the second and third cycles, a capsule containing 500 mg of RP was given to group one, a 500-mg capsule containing Favania to the experimental group 2 and capsule containing 250 mg of Mefenamic acid in 500 mg capsule sachets to the third group. The results of this study showed that Rivand (RP) significantly reduced the severity and duration of menstrual pain compared to the other two drugs (p<0.001(. Also, the volume of menstrual bleeding in these this group was significantly reduced, which was less than that of the Mefenamic acid group (p> 0.05). Blood volume in the Fovania group was significantly higher in comparison with the other two groups (p <0.001). The results of this study showed that RP significantly decreased the severity and duration of menstrual pain and the volume of bleeding during menstruation. No major side effects have been reported, therefore, it can be a good alternative to treatment of primary dysmenorrhea in patients.
Effect of Crocus sativus stigma extract on cognitive criteria, anti-inflammatory and antioxidant markers in Nesyan patients (Alzheimer's disease)
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2022) Rasi marzabadi, leila; Araj-Khodaei, Mostafa; Sadigh-Eteghad, saeed; Traditional medicine; talebi, mahnaz; Fazljou, Seyyed Mohammad Bagher
Background: Mounting evidence indicates the possible positive effects of Crocus sativus (saffron) on cognitive function in patients with Alzheimer’s Disease (AD). This study assessed the effects of saffron on cognitive outcomes and circulatory markers in patients with mild-to-moderate AD. Methods: Sixty adults with mild-to-moderate AD were found eligible to take part in a 12-week, randomized double-blind placebo-control clinical trial with a parallel-group design. Volunteers were randomized to receive donepezil plus either saffron capsule at 15 mg/twice a day dose or placebo (n=30 each). The main outcomes of this study were the changes in the mini-mental state examination (MMSE) score, serum inflammatory markers (IL-1β, IL-6, and TNF-α), and serum antioxidant and oxidative stress status markers (glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA), and total antioxidant capacity (TAC)). Results: MMSE score of the patients in the saffron group was not significantly different from the placebo group (p=0.303). When compared to the placebo treatment, saffron administration significantly decreased IL-1β (p=0.036) and MDA (p=0.021) levels and increased TAC (p=0.032) in the serum sample of mild-to-moderate AD patients. Conclusion: This study showed no additional effects of saffron supplementation on cognitive outcomes in donepezil-treated mild-to-moderate AD patients; however, patients in the saffron group had improved inflammatory, antioxidant, and oxidative stress profiles compared with the placebo group, which may prove the beneficial effects of the additive on circulatory markers in AD patients.
Effect of curcumin on transcriptomics profile of breast cancer cells: possible relation with telomerase gene expression
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2022) Khazei, Keyvan; Torbati, Mohammadali; Persian Traditional Medicine; Zarghami, Nosratollah; Fazljou, Seyyed Mohammad Bagher
Abstract A systemic approach provides a comprehensive scheme from the regulatory molecular mechanism of drugs in living systems. In this study, we investigated the regulatory mechanism of Curcumin in breast cancer cells by analyzing the transcriptome alterations in the T47D cell line. The cytotoxic effect of Curcumin was investigated by MTT assay after the treatment of T47D cell lines with 0-50 µM concentration of Curcumin. Furthermore, transcriptome analysis was performed using RNA sequencing to find differentially expressed genes following Curcumin treatment in T47D cells. Transcription factor and microRNA profiles related to Curcumin gene signature retrieved from related databases. A system-based network analysis performed to deepen our understanding of the Curcumin mechanism of action in T47D cells. In an independent analysis, a publicly available microarray dataset including gene expression and clinical data used to evaluate the prognostic power of Curcumin signature and its relation with patients’ outcomes by the Cox model. Our results suggest that Curcumin triggers cell death-related pathways and regulates the metabolism of lipids and apoptotic process in T47D cells. Curcumin signature had significant prognostic power. Regulatory network analyses found specific proteins, microRNAs and transcription factors as the hub nodes which corresponded to the effects of Curcumin treatment. This work provides a picture of the cytotoxicity mechanism of Curcumin and potential therapeutic targets for treatment optimization of breast cancer.
Effect of Echium amoenum Hydroalcoholic Extract on Restraint Stress-induced Depressive- and Anxiety-like Behaviors in Mice
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2019) Nouri, Mohammad; Hamedyazdan, Sanaz; Sadigh-Eteghad, Saeed; Ranjbar, Fatemeh; Traditional Medicine; Araj-khodaei, Mostafa; Torbati, Mohammadali
Abstract Introduction: Depression is a threatening disease. Due to adverse effects of chemical antidepressant drugs, researcher’s attention has been shifted toward natural drug. Objective: This study was designed to investigate the effect of Echium amoenum (E. amoenum) treatment on anxiety- and- depressive-like behaviors as well as neuroinflammation and hepatotoxicity markers in restraint stress (RS)-subjectedmice. Methods: Phytochemical properties of E. amoenum extract analyzedby evaluation of total phenolic, flavonoid, rosmarinic acid levels and radical scavenging activity. For behavioral studies, animals were randomly assigned into five groups as follows: control, RS, RS+E75, RS+E150, and RS+E300. Animals in the RS group were subjected to the RS (3 h/day for 14 days) and treated with normal saline, while treatment groups were received different doses of E. amoenum (75, 150, and 300 mg/kg, P.O.) concomitantly with RS exposure. At the end of the period, behavioral tests were used to evaluate depression and anxiety.Moreover tissue samples from the prefrontal (PFC), hippocampus (HIP) and liver were performed to assess protein expressions of NF-κB, TNF-α, IL-1β, and IL-6) and liver histology and blood samples for the evaluation of corticosterone, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alanine phosphatase (ALP). Findings: E. amoenum increased percentage of presence in the central area of the Open Field Test, percentage of frequencies and spent time in open arms of Elevated Plus Maze. Also it decreased the immobilization time in the Tail Suspension and Forced Swimming Tests. These results were accompanied by decreased serum corticosterone levels. Furthermore, E. amoenum decreased protein expression of neuroinflammatory markers in the HIP and PFC subregions. Although RS slightly increased serum levels of liver injury markers, no histopathological changes were seen in the RS or E. amoenum-treated groups. Conclusion: These findings suggest that E. amoenum can be an effective and safe complementary strategy for the treatment of stress-associated biochemical and behavioral changes. Keywords: Restraint stress, Anxiety, Depression, Neuroinflammation, Echium amoenum, Corticosterone,
The effect of Iranian Traditional Medicine treatment based on Withania somnifera on sperm parameters in the infertile men: A Randomized, Triple-Blind, controlled clinical trial
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2018) Nasimi Doost Azgomi, Ramin; Nazemyieh, Hossein; Sadeghi Bazargani, Homayoun; Nejatbakhsh, Fatemeh; Traditional medicine; Zomorrodi, Afshar; Fazljou, Seyed Mohammad Bagher
Withania somnifera (WS) is a medicinal herb that is used in Persian medicine to treat male infertility. Thus, we aimed to assess and compare the effects of WS and pentoxifylline on the sperm parameters in idiopathic male infertility. This study was a triple-blind randomized clinical trial. 100 infertile male patients were randomly allocated into either WS or pentoxifylline groups. Patients in the herbal group received 6 capsules containing 5 g/daily of WS root and in the pentoxifylline group subjects received 6 capsules containing 800 mg/daily of pentoxifylline and placebo for 90 days. Sperm parameters were analyzed at the beginning and end of the study. Results showed that WS administartion significantly increases mean sperm count (12.5%) as well as progressive motility (21.42%) and improved sperm morphology (25.56%) compared to the baseline (p=0.04, p=0.001 and p=0.000, respectively). We also showed that pentoxifylline treatment remarkably increased mean semen volume (16.46%) as well as progressive motility (25.97%) and improved sperm morphology (13.28%) compared to the baseline (p=0.02, p=0.003 and p=0.01, respectively). Intergroup comparison showed no statistically significant differences between two groups on the sperm parameters after 90 days. No major complication was reported either in WS or PTX groups. WS and pentoxifylline both improve sperms parameters in idiopathic male infertility. However, the impacts of two treatments were not different. Due to no noticeable adverse effects, this medication can be an alternative choice in the treatment of idiopathic male infertility.
The effect of sericin on anxiety, depressive-like behaviors, oxidative stress as well as apoptosis induced by chronic restraint stress in male mice
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2019) Banagozar Mohammadi, Ahad; Sadigh-Eteghad, Saeed; Fazljou, Seyyed Mohammad Bagher; E.J. Golzari, Samad; Traditional Medicine; Mahmoudi, Javad; Torbati, Mohammadali
Introduction: Stress, depression and anxiety are now considered as the most prevalent disease in the century. Long-term exposure to stress through different mechanisms causes complications and consequences. Prevention of destructive effects of stress and providing appropriate preventive and therapeutic methods are of illicit drug importance. Objective: Since years away in traditional Iranian medicine, the therapeutic uses of silk are especially common for the treatment of depression, memory and brain function enhancement. Considering the traditional medicine literatures on the insure of silk effect in a wide range of brain diseases, in this study, the protective effects of sericin as the main protein of silk is investigated in prevention of stress-induced brain complications. Methods: Animals were subjected to chronic inhibitory stress for 3 hours and within 21 days sericin were administrated at doses of 100, 150 and 200 mg/kg/day. At the end of the period, behavioral tests were used to evaluate depression and anxiety. Then, blood samples were obtained to measure serum corticosterone level and prefrontal cortical as well as hippocampal samples were used to evaluate the level of oxidative stress and apoptosis markers. Results: Sericin increased percentage of presence in the central area of the Open Field Test, percentage of frequencies and spent time in open arms of Elevated Plus Maze. Also it decreased the immobilization time in the Tail Suspension and Forced Swimming Tests. Sericin administration decreased lipid peroxidation levels and increased total antioxidant capacity (TAC) and GPx and SOD activities in the prefrontal cortex and hippocampus. Sericin modulates serum corticosterone concentration and inhibits apoptosis by decreasing Bax to Bcl-2 ratio and decreasing Caspase 3 levels in these regions. Results: The findings indicate the protective effect of sericin in the face of chronic restraint stress and prevention of behavioral, psycho-pathological and molecular changes.
The effect of Urtica Dioica(Nettle) and Vaccinium myrtillus on blood glucose parameteres and lipid profile in patients with type 2 diabetes: a double-blind clinical trial
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2022) chehri, anita; shakouri, seyed kazem; ostad rahimi, alireza; yarani, reza; Traditional Medicine; mobasseri, majid; Araj-Khodaei, seyed mostafa
Abstract Introduction: Type 2 diabetes (T2DM) is a chronic disease characterized by an imbalance in glucose metabolism in the body. Type 2 diabetes is the most common type of diabetes. Due to the high prevalence of diabetes and its complications, as well as the complications of chemical drugs, physicians are looking for alternative drugs, especially medicinal plants, to treat diabetes. Urtica Diocia and Vaccinium Myrtillus are among the plants that have many uses in Iranian traditional medicine. One of the uses of these two plants is in treating diabetes. Aim: Determining the effect of Urtica Diocia and Vaccinium Myrtillus on blood sugar parameters and fat profile in patients with type 2 diabetes. Methods: The present study was a double-blind, randomized controlled clinical trial. Sixty patients with type 2 diabetes were randomly selected based on inclusion criterias and after obtaining informed consent included in the study. The patients were randomly assigned to two groups by random block method: 1- receiving Urtica Diocia and Vaccinium Myrtillus 2- receiving placebo. Patients consumed the drugs for three month. Demographic, medication side effects, quality of life in diabetic patients and Mezaj questionnaires, blood pressure measurement, wrist and waist circumference, weight, height, etc., completed and fasting blood samples were performed before and after the intervention. Results: The statistical comparison of the two groups showed a significant difference in fasting blood sugar levels in the two studied groups (p < 0.001). After the intervention, the average reduction of triglycerides in the patients of the Urtica Diocia and Vaccinium Myrtillus group had a significant difference from the average decrease of this parameter in the placebo group (p < 0.001). Also, the combination of Urtica Diocia and Vaccinium Myrtillus reduced cholesterol significantly compared to the placebo (p < 0.001). Urtica Diocia and Vaccinium Myrtillus significantly controlled and reduced LDL compared to placebo (p < 0.001). Conclusion: The results of this study showed that the combination of Urtica Diocia and Vaccinium Myrtillus could play an effective role in reducing blood sugar and fat profile in patients with type 2 diabetes.
The effect of walnut-fig syrup in geriatrics constipation in comparison with the lactulose: A double blind randomized clinical trial
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2022) Joudikhajeh, Saeed; Mehdizadeh Esfanjani, Robab; Traditional medicine; Araj- Khodaei, Mostafa; Sepehri, Bita
Introduction: Functional constipation (FC), or chronic idiopathic constipation, is one of the most common chronic disorders with no specific cause. Although FC is not associated with the progress of severe disease or additional mortality, it significantly decreases the patients' quality of life (QoL). While several medications are available to treat constipation, there is no adequate treatment response in most cases. Herbal remedies are one of the most widely used options for FC. Methods: In this double-blind controlled trial, all patients, researchers, and analysts were unaware of the ingredients contained in the drugs, as well as the assigned groups. We recruited a total of 90 older adults patients diagnosed with FC according to the Rome IV Diagnostic Criteria for Irritable Bowel Syndrome (IBS). Patients were divided into two equal groups (n=45 in each) receiving Walnatin syrup or lactulose syrup. Then, CBC-FBS-TSH-CR-NA-K-CA tests were taken from all patients, and the developed syrups (Walnatin or lactulose) were prescribed to patients (15 cc half an hour before bedtime). Results: While 43 (95.6%) patients responded to treatment in the intervention group, 41 (91.1%) patients responded to treatment in the conventional treatment group. There was no statistically significant difference between the two groups in terms of response to treatment (P = 0.677). Conclusion: The results showed that the oral syrup developed in this study improved FC in the elderly and had no side effects.
Effects of Pistacia lentiscus and Coriander Triphala on Adult Gastroesophageal Reflux Disease: A Randomized Double -Blinded Clinical Trial
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2020) Sadeghi, Fariba; Khodaei, Laleh; Monirifar, Hassan; Traditional Medicine; Fazljou, Seyed Mohammad bagher; Sepehri, Bita
Abstract Background: Gastroesophageal reflux is one of the most common gastrointestinal diseases. Its prevalence in Iran has been reported from about two to 50 percent. The cardinal symptoms of gastroesophageal reflux disease include heartburn (pyrosis) and regurgitate in to the throat and other nearby organs. In Persian medicine, several drugs have been mentioned for the treatment of gastrointestinal diseases. Objectives: This study aimed to assess the effects of Pistacia lentiscus (Mastic) and Coriander Triphala on reflux symptoms compared to omeprazole in a double-blinded randomized clinical trial. Methods: In a double-blinded, multicenter, randomized clinical trial, we assessed the effects of Pistacia lentiscus L., Coriander Triphala and omeprazole on the symptoms of GERD in Tabriz, Iran, in 2018-2019. Participants were randomly divided into three groups as group A (Pistacia lentiscus L, 1000 mg/TDS), group B (Coriander Triphala, 1000 mg/TDS) and group C (Omeprazole, 20 mg/day plus five placebo capsules per day). The assessments were done at the beginning and the end of the study using FSSG, VAS, RS and GERD-HRQL questionnaires Results: In the beginning, no significant differences were observed between the groups in the background characteristics. There was no statistically significant difference between Pistacia lentiscus, Coriander Triphala, omeprazole in the improvement of FSSG, VAS, GERD - HRQL and reflux scores. In all groups, the FFSG, VAS, reflux and GERD-HRQL scores significantly decreased and improved after four weeks of intervention compared to the respective baselines. The FSSG score improvements after four weeks of intervention were 77.24%, 75.40% and 65.36% in groups A, B, and C, respectively (P=0.000). The VAS score improvements were 66.34%, 68.78%, and 58.50% in groups A, B and C, respectively (P=0.000). Improvements in GERD-HRQL were 86.88%, 82.19% and 74.47% in groups A, B and C, respectively (P=0.000). Reflux improvements were 77.21%, 72.20% and 65.57% in groups A, B and C, respectively (P=0.000). Conclusion: The results showed that Pistacia lentiscus and Coriander Triphala are as effective as omeprazole in the treatment of GERD. Herbal medicines can be used to treat gastrointestinal diseases with fewer side effects and more benefits.
Evaluating the healing effect of Ajuga chamaecistus tomentella on second grade burn wounds in patients referred to burn center
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2022) Rahiminiya, Aysan; Salehi, Seyed Hamid; Shirbeigi, Laila; Fazljou, Seyyed Mohammad Bagher; Faculty of Traditional Medicine; Herizchi Ghadim, Hamideh; Ayati, Mohammad Hossein
Evaluating the Healing Effects of Ajuga chamaecistus ssp. tomentella, on Second Grade Burn Wounds, a Double-Blind Randomized Controlled Clinical Trial Abstract Background: Nowadays, the use of medicinal plants is on the rise mainly due to its fewer side effects and fewer treatment costs. Despite the traditional reports on the healing property of Ajugaspecies, no clinical studies have been conducted. In this study, the effect of hydroalcoholic extract cream of Ajugachamaecistusssp.tomentella (3%) had been compared with nitrofurazone cream (0.2%) on the second-degree burn wound healing. Materials and Methods: This clinical trial was performed at Motahhari Burns Hospital in Tehran. Fifty-two cases of second-degree burn patients were randomly assigned to two groups of 26 members, including nitrofurazone and Ajuga, and were bandaged daily. This study evaluated wounds based on the onset of epithelialization, healing time, post-drug irritation, primary irritation, decreased irritation, post-drug pain, primary pain, decreased pain,allergy, infection parameters, and the bates-jensenWound Assessment Tool. SPSS, version 20 were used for data analysis. Results: Based on the observed results, Ajuga cream was compared to nitrofurazone in cases of the mean day of epithelialization onset (P-value = 0.007), healing time (day) (P-value = 0.001), Post-drug irritation (P -value = 0.007), decreased irritation (P-value <0.001), post-drug pain (P-value = 0.018), decreased pain (P-value = 0.001) (all with P value less than 0.05); it was observed that Ajuga was more effectivethannitrofurazoneand there was a statistically significant difference between the two types of drugs. According to the results, the Ajuga cream (3%) can be a useful remedy for burn wounds due to the reduction in the onset of epithelialization, healing time, post-drug irritation, irritation reduction, post-drug pain, and pain reduction. However, further large clinical trials are needed to confirm these results.
Evaluation of combination anti cancer effect of methotroxate and melissa officinalis as a herbal component on HeLa cancer cell line
(Tabriz University of Medical Sciences, School of Traditional Medicine, 2022) Faraji, Parviz; Hamedeyazdan, Sanaz; Mohammadinasab, Reza; Traditional medicine; Ezzati Nazhad Dolatabadi, Jafar; Araj-Khodaei, Mostafa
Background and objective: Cervical cancer is the second most common cancer after breast cancer among women worldwide. Conventional treatments for this disease include surgery, radiotherapy and chemotherapy. Among the above methods, chemotherapy is currently used significantly, but the use of chemotherapy drugs has undesirable side effects. Simultaneous administration of two or more chemotherapy agents or conventional drugs as a combination therapy is the most effective way to increase the effectiveness of treatment. Additive or synergistic effects are two mechanisms by which combination therapy increases the optimal treatment of cancer compared to a single treatment. One of the accepted beliefs about natural compounds as herbs is their effectiveness in treating cancer. However, in most cases, its effects in clinical phases have not been studied accurately and logically. Therefore, it may be necessary to study the anticancer effects of compounds such as MO and to determine the mechanisms involved in cancer. In this study, HeLa cancer cells were used to evaluate the anti-cancer effect of MO in combination with MTX on growth retardation, induction of apoptosis, morphological changes and fractures in the DNA of cell nuclei and to compare the therapeutic effects of this combination with MO and MTX alone. Materials and Methods: MTT assay assessed the quantitative cytotoxicity effect of both MTX and MO. Apoptosis assay via flow cytometry was used to determine the amount of apoptotic and necrotic cells. To further investigate the anti-cancer effects, DAPI staining and DNA ladder assays are used qualitatively to detect changes in the nuclei of cells that are a sign of apoptosis occurring and morphological modifications of DNA. Results: MTX and MO mixture showed high cytotoxicity and apoptosis rate compared to untreated cells. Furthermore, the morphological changes of MTX and MO mixture were more evident than that of single MO, MTX, and control groups. Conclusion: These data regarding cell growth reduction and apoptosis induction in HeLa cancer cells showed that MTX and MO mixture can be an appropriate platform for cancer therapy.