Theses(M)

Permanent URI for this collectionhttps://dspace.tbzmed.ac.ir/handle/123456789/2

Browse

Filters

2019 - 201912020 - 20221

Settings

Author: search.filters.author.Abbaszadeh, Sina

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item type: Item ,
    Downregulation of BHRF1 in chronic lymphocytic leukemia cells in order to enhance apoptotic effects of doxorubicin
    (Tabriz University of Medical Sciences, Faculty of Medicine, 2022) Abbaszadeh, Sina; Shahmohammadi Farid, Sima; JadidiNiaragh, Farhad; Ghalamfarsa, Ghasem
    Resistance of leukemic cells to apoptosis is one of the most important problems in the treatment of various leukemias. Chemotherapy drugs such as doxorubicin have been routinely used in the treatment of leukemias such as chronic lymphocytic leukemia (CLL), which have unfortunately been associated with low efficacy. Therefore, one of the ways that can be used to treat leukemias is to inhibit the ways of resistance to apoptosis in leukemic cells. One of the important factors that plays an important role in resistance to apoptosis in malignant cells is BHRF1 factor, which is homologously Bcl-2 homologous and leads to resistance of leukemic cells to programmed cell death. Therefore, in this study, we decided to reduce their susceptibility to doxorubicin-induced cell death by inhibiting BHRF1 expression in CLL cells. For this purpose, we used chitosan-based nanoparticles to transfer BHRF1 siRNA and doxorubicin to leukemic cells. Materials and Methods: In this study, carboxymethyl dextran coated trimethyl chitosan nanoparticles were used for simultaneous delivery of BHRF1 siRNA and doxorubicin to leukemic and normal cells. H1NMR and FTIR tests were used to study the chemical structure of the synthesized nanocomplexes. The physicochemical properties of the nanoparticles were also evaluated by DLS. SEM microscope was also used to study the morphology of the produced nanoparticles. Also, the entry of nanoparticles into leukemic cells was examined by confocal microscopy and flow cytometry. Toxicity assessment of nanoparticles loaded with siRNA and doxorubicin molecules was also assessed by MTT assay. The expression of the desired genes was also examined using real-time PCR technique. Flow cytometry was also used to evaluate apoptosis in leukemic and normal cells. Results: Physicochemical analysis of the produced nanoparticles showed that they were about 105, zeta potential 18 and PDI <0.2 and could load siRNA and doxorubicin well and deliver them to malignant cells. Cytotoxicity showed that the nanoparticles did not have much toxicity on the cells, while nanoparticles loaded with siRNA and doxorubicin significantly caused cell death. In addition, co-administration of BHRF1 siRNA and doxorubicin to cells induced apoptosis in cells.
  • Thumbnail Image
    Item type: Item ,
    Evaluation of Thyroid function profile in patients with major depressive disorder
    (Tabriz University of Medical Sciences, Faculty of Medicine, 2019) Abbaszadeh, Sina; Shafiee, Alireza; Farhang, Sara; Ghaemmaghami, Mehrdad
    The purpose of this study was to determine the thyroid function profile in patients with major depressive disorder. Materials and Methods: For all patients with entry criteria, if completed, the Hamilton Depression inventory was filled in at the first visit, which would determine the severity of depression. At the same time, TSH, Free T4 and anti TPO tests were requested, all performed as a fasting blood sample from an environmental vein and in a single laboratory (under the supervision of the university). On the other hand, the relationship between the reported hormonal head and severity of depression (Pearson correlation) was also investigated. Results: In this study, 100 patients with major depression were evaluated. The mean ± standard deviation of the patients' age was 35.38±9.39 years. 29 cases (29%) were males and 71 cases (71%) were females. Mean ± standard deviation of TSH values in all patients was 2.80 ± 2.35 mIU/L; mean±SD of FT4 values in all patients was 14.21±3.35 pmol/L; and mean±SD of anti-TPO values in all patients, 46.46±70.84 IU/mL was. Mean ± SD for severity of depression at the beginning of the visit was 20.32±5.49 and after the 4 weeks of treatment, it was 8.69 ± 5.27 which was significantly decreased (p=0.001). There was a significant correlation between severity of depression and TSH and FT4, direct correlation and anti-TPO.