Isolation and Characterization of aquatic Bacteria of Quri-Gol lake in 2021

Abstract

Qurigol is one of the most important wetlands in East Azarbaijan province in terms of non-pathogenic bacterial population. To identify beneficial microorganisms, we referred to Qurigol place to collect, isolate and characterize the bacterial populations.Objectives: The objectives of this research were to isolate the bacteria present in Qurigol and then determine their identity with respect to related species and to investigate phenotypes such as gram, oxidase,Methodology: During the isolation process, the bacteria were cultured as single colonies separated from each other. Finally, after passing through the DNA extraction stage, phenotypic examination including gram test, oxidase, urease, catalase and motility was examined.Results: In this study, the bacteria isolated from the Qurigol pond were isolated and phenotypically investigated. After DNA isolation and extraction, 7 isolates were sent for 16srRNA sequencing due to having suitable optical absorption and the results of phenotypic tests were also reported. According to the results, the majority of these bacteria were gram positive, catalase positive, KOH negative, oxidase positive and amylase negative. Conclusion: Observing the state of the Qurigol showed that the dryness of the environment has damaged the normal flora, and the same can be concluded from the findings. We hope that in the coming years more importance will be given to valuable resources and measures will be taken to recover the area.