Investigation of chemotherapy drugs effect on immune checkpoints expression in the cervix cancer cell line

Abstract

The study aims to understand how Docetaxel and Doxorubicin, two commonly used chemotherapeutic drugs, influence the expression of PD-L1, CTLA-4, and VISTA in cervical cancer cells. Method: In this study, The CASki cell line was cultured using standard cell culture techniques. The MTT test was employed to ascertain the half-maximal inhibitory concentration (IC50) and potency of chemotherapeutic agents Docetaxel and Doxorubicin. We used qRT-PCR to investigate the expression of PD-L1, CTLA-4, and VISTA genes in the CASki cell line after treatment. Result: Doxorubicin significantly increased the expression of PD-L1 (P-value < 0.05) and CTLA-4 (P-value < 0.001) and decreased the expression of VISTA (P-value < 0.01) in the CaSki cell line, but Docetaxel increased the expression of PD-L1 (P-value < 0.001), CTLA-4 (P-value < 0.001), and VISTA (P-value < 0.05).