Assessment of the Falcarindiol Effect against Cisplatin-Induced Nephrotoxicity in Male C57/BL6 Mice

Abstract

cisplatin is a comotherapy drug for the treatment of solid tumors. The drug has various effects, the most important of which are peripheral neuropathy, nephrotoxicity and Ototoxicity. The aim of this study is to investigate the protective effect of Falcarindiol against cisplatin-induced nephrotoxicity in male mice C57/BL6. Materials and methods: to study their Falcarindiol, 30 5-week-old male C57/BL6 mouse were divided into 5 groups of 6 of healthy control groups, cisplatin group, Falcarindiol group (two doses) + cisplatin group and Falcarindiol group. 12 hours after the last injection of the desired composition, blood was drawn from the mice in accordance with animal protocols, and the serum of the mice was isolated by centrifuge and kept in the freezer-80 for urea and Creatinine Tests until the day of the test, and after the blood was taken, the mice were killed immediately and their kidney tissue was removed for Molecular, protein and histological examinations. Findings: falcarindiol compounds meaningfully (p<0.001) reduce the serum content of urea, creatinine, which, following this decrease, also reduces the tissue content of the NFkB, MAPKs pathway intermediates, which inhibits inflammation and reduces the amount of apoptosis. The combination of Falcarindiol also causes the production of antioxidants and suppression of inflammatory cytokine TNF - α by activating AMPK and subsequently inducing the Nrf2 signaling pathway.