Study on carbapenemases activity in MDR Klebsiella pneumonia isolates by mCIM method and carbapenemase genes blaVIM , blaIMP, blaNDM , blaKPC-2 and blaOXA-48

Abstract

Klebsiella ‌pneumoniae is a Gram-negative bacterium. Carbapenem drugs are used to treat cephalosporin-resistant Klebsiella ‌pneumoniae. The emergence of MDR (multi-drug-resistant) Klebsiella‌ pneumoniae isolates is a serious limitation and can be an alarm for the failure of common treatments. The aim of this study was to investigate the activity of carbapenemases in Klebsiella‌ pneumoniae isolates by mCIM method and carbapenemase genes. Materials and Methods: In this study, the ability to produce carbapenemase in MDR Klebsiella ‌pneumoniae isolates was determined by the mCIM method and the presence of carbapenemase genes was investigated by PCR. Results: The results showed that among 100 MDR Klebsiella pneumoniae isolates, 80 isolates were mCIM positive and have the ability to produce carbapenemase. Also, among 80 positive mCIM isolates, PCR showed that 52 isolates had blaOXA-48 gene, 23 isolates had blaNDM gene, 12 isolates had blaIMP gene, 8 isolates had blaVIM gene, 5 isolates had blaKPC-2 gene.