The effect of resveratrol on increasing the sensitivity to doxorubicin through oxidative DNA damage in osteosarcoma cancer cells

Abstract

Recently, various studies have focused on the therapeutic potential of resveratrol in various human malignancies, including osteosarcoma. However, the underlying mechanisms in resveratrol-mediated anticancer effects are still not fully understood. Therefore, the present study investigated the effect of resveratrol on doxorubicin-induced apoptosis through DNA damage in SAOS-2 cells. Methods SAOS-2 cells were treated with doxorubicin, resveratrol and a combination of both, and cell viability was evaluated by MTT method. The expression levels of DNA damage markers were evaluated using qRT-PCR. Apoptosis was also investigated by flow cytometry. Results Doxorubicin resulted in significant inhibition of cell proliferation in a dose-dependent manner. The combination of doxorubicin and resveratrol resulted in significant inhibition of proliferation compared to single treatments (P<0.05). Resveratrol also induced apoptosis through increased expression of H2AX, ATR, ATM, Rad51, and p53. Furthermore, resveratrol increased doxorubicin-induced apoptosis in SAOS-2 cells.