Investigating the impact of simultaneous silencing of STAT3 and IGF-1 with SPION-HA-PEG nanoparticle in 4T1 and CT26 cancer cells

Abstract

The growth and spread of cancer cells in the tumor microenvironment is influenced by several factors. Many factors in the tumor area increase proliferation and survival and suppress antitumor immune responses. STAT3 and IGF-1 factors are among the most important factors whose expression is increased in cancer cells and can significantly increase the survival, proliferation, angiogenesis, and metastasis of cancer cells. On the other hand, they play an important role in suppressing immune responses. As a result, the combined inhibition of these two factors can be considered an important method in an effective method in cancer immunotherapy. Materials and methods: This study was conducted on two mouse breast cancer cell lines T14 and CT26 mouse colon cancer. In this study, nanoparticles loaded with siRNA molecules were used to suppress the expression of STAT3 and IGF-1 factors in cancer cells. After the treatment of the cells, the effect of the combined treatment on the survival of the cells was evaluated using the MTT test and its effect on the survival of the target genes was evaluated using the Real-time PCR test. Results: The results of this study showed that nanoparticles loaded with siRNA could effectively reduce the expression of STAT3 and IGF-1 factors in both cell lines. Suppression of the expression of these factors led to a decrease in the survival of both cell lines. Also, inhibiting the expression of STAT3 and IGF-1 factors led to a decrease in the expression of genes involved in cell survival, angiogenesis, metastasis, and cell proliferation.