Evaluation of the effects of exosomes derived from human stem cell mesenchymal cells (MSCs) on differentiation and function of 17 helper T cells (Th17) in patients with chronic obstructive pulmonary disease (COPD)

Abstract

Inflammatory responses play a significant role in the development and progression of COPD. Recent studies have shown. So far, various treatment methods have been proposed to treat these patients. In this study, we investigated the regulatory effects of exosomes derived from MSC cells on the activity of peripheral blood Th17 cells of patients with COPD. Methods and Materials: In this fundamental study, in order to isolate PBMC cells, 8 ml of blood containing anticoagulant was taken from 20 COPD patients. After sampling and separating PBMC cells from them, the differentiation percentage of TH17 cells and the level of gene expression and secretory level of cytokines IL-17, IL-22, Il-23 before and after co-culture with derivative exosomes. It was measured from MSC cells. Finally, Graphpad Prism software was used for data analysis, and p>0.05 was considered as a significant level. Results: The results of our study showed that the secretion of cytokines IL-17, IL-22, IL-23 significantly decreased in PBMC of patients after co-culture with exosomes derived from MSC cells. Also, the expression level of RORγt, IL-17, IL-22 and IL-23 genes has decreased significantly. In addition, a significant decrease in the differentiation rate of lymphocytes to TH17 was observed after co-culture with MSC exosomes.