Effect of moderate exercise on cyclosporine-induced liver damage via AMPK/ sirt-1/ and autophagy pathway in male rat liver

Abstract

Drug-induced liver damage is an important cause of liver diseases. In this study, we aimed to investigate the effect of exercise on the liver damage caused by cyclosporine A through the AMPK/sirt-1 signaling pathway and autophagy in the liver tissue of male rats. Materials and Method 24 male Wistar rats with a weight range of 220 ± 10 grams were randomly divided into three groups of 8: 1) control group 2) cyclosporine group (30 mg/kg/po): the drug was gavaged daily for six weeks. 3) cyclosporine + moderate exercise group: at the same time as receiving the drug, they underwent an exercise protocol for six weeks. Then the animals were anesthetized and the chest was opened and blood was taken directly from the heart. After blood collection, the liver was isolated and used to measure the levels of AMPK, Sirt-1, Beclin-1 and LC3II/I by western blot method. Results: This results show that exercise a significant effect on the morphology of the damaged liver. Sirt-1 levels significantly decrease in liver tissue after cyclosporine consumption. In the main group, after six weeks of exercise, the amount of this parameter increased significantly and almost reached the level before receiving the drug. On the other hand, even though the amount of LC3 in the liver tissue after the injury caused by the drug was not much different than before the drug, after six weeks of exercise, the amount of this parameter was significantly increased in the liver tissue. Also, in the case of Beclin-1, the effect of the drug on the liver caused a significant increase in the liver tissue, but despite this, exercise did not cause a significant decrease in this parameter.