The prevalence of Candida species in gingival crevicular fluid in patients with chronic periodontitis referred to the dental faculty of Tabriz University of Medical Sciences

Abstract

Gingival Cervical Fluid (GCF), which is adjacent to periodontal tissue, is used to investigate proteomic changes related to periodontal disease. It has been reported that small amounts of GCF are present in healthy gingiva, while large amounts are observed in inflamed areas. This study aimed to isolate and investigate the abundance of Candida species in the gingival crevicular fluid of patients with chronic periodontitis referred to the dental clinic affiliated with Tabriz University of Medical Sciences by microscopic and molecular method. Materials and Methods: In this project, Candida species were isolated from susceptible and chronic periodontitis patients in the dental clinic of Tabriz University of Medical Sciences, and after being referred to the specialized mycology laboratory of the Faculty of Medicine, they were identified by microscopic and molecular methods. And the abundance of Candida species was determined. To determine Candida species, the isolation area was first determined and sampling was done from subgingival biofilm and gingival crevice fluid. To identify the molecular method after extracting the DNA of Candida isolates, using ITS1 and ITS4 primers, ITS1-5.8S-ITS2 rDNA regions were amplified by PCR, and after confirming the formation of the expected bands, using restriction enzymes MspI was determined by molecular method by PCR-RFLP method. Results: The results of the identification of Candida clinical strains by CHROMagar, PCR-RFLP, and Sequencing techniques showed that 12 samples are related to C. albicans, C. glabrata, 5 samples are related to C. krusei, 1 sample is related to C. dubliniensis, 1 sample related to C. tropicalis and 1 sample related to C. pararugosa.