The effect of cordisipin on the expression of gastric cancer cell metastasis genes

Abstract

Metastasis is considered as an important hallmark of cancer progression. The MMP2 and talin2 genes are among the genes that progress the angiogenesis. They are repressed by miRNAs that control them and may also be used as part of treatment-responsive biomarkers. The aim of this study was to investigate the expression of these genes and miRNAs that control them in treatment with cordycepin. Material and Methods: MKN-45 gastric cancer cell line was cultured in the relevant culture medium and after treatment with cordycepin, RNA was extracted. Then cDNA was synthesized. Finally, changes in the expression of MMP2, talin2 genes and their control miRNAs were determined by qRT-PCR. Results: QRT-PCR test changes in the expression of MMP2, and talin2 genes decreased significantly, and miRs controlling them, miR-194, in treatment with cordycepin showed a significant increased.