Charactrrization of human platelet lysate in gene expression profile of growth factors on human mesenchymal stem cells

Abstract

Stem cell transplantation has had several therapeutic successes over the last three decades and has turned into a practical treatment for several cancers, anemia, leukemia, and immunological disorders. Two populations of stem cells exist including Hematopoietic stem cells (HSC) and Mesenchymal stem cells (MSC). HSCs are CD34+ and can produce all three blood cell lineage including red blood cells, white blood cells, and platelets. MSCs are CD29+, CD44+, CD73+, CD90+, CD105+ and are important in proliferation and differentiation of HSCs. MSCs can be obtained from several sources including bone marrow, adipose tissue, lung, liver and amniotic fluid of fetus. However, BM and adipocyte sources are more common. Isolation of these cells from humans is trivial with minimum side effects. Therefore these cells have become an attractive source for cell therapies. MSCs comprise rare population of BM mononuclear cells therefore to harverst proper number for transplantation should be extensively expanded. Traditionaly FBS were used as an supplyment to support MSC expansion albeit worries about this animal products have always been existed. Mesenchymal stem cells exert their therapeutic functions mostly through the secretion of various cytokines and growth factors. minimum change in culture component would affected the MSC-Derived secretome. In the present study, we have examined media including allogenic human platelet lysate (HPL) or FBS in order to compare the gene expression profiles of some these products Materials and methods: in this study BM-MSCs were cultured in two T25 flasks containing different media supplemented T= HPL 5% and C= FBS 10%, MSC markers were evaluated using flow cytometry then total RNA isolated from MSC and cDNA was synthesized. The amplification pattern of growth factors and cytokines such as SCF1- FGF2- CCL28 - IL1a - IL 11- IL12 A, IL-14 IL17RA- IL-8 -NT3- IL-6- IGFBP ANG- TGFb3- SDF2 - IL10, IL-15 VEGFB-LIF-GM CSF-oncostatin M- OPG- HGF- MCP were compared in both Test (PL) and control group(FBS) using Real Time PCR method. Result: This study shows that hPL can totally support MSCs expansion and also induce changes in the portion of certion products.