Targeting colorectal cancer cells using silibinin loaded on magnetic niosomes

Abstract

In recent decades, the appearance of different nanoparticles (NPs) has revolutionized targeting drug delivery in cancer cell treatment. Niosomes are a type of NPs used in drug delivery and consist of layered structures of vesicles made from non-ionic surfactants. Recently, magnetic niosomal NPs (MNNPs) conjugated with anticancer drugs are investigated. Promising results are reported about stimulus-responsive drug release at the target tumor site under the influence of an external magnetic field. This research aimed to investigate the cytotoxicity property of silibinin-loaded magnetic niosomes nanoparticles (MNNPs) in colorectal cancer cells. Methods: the co-precipitation method was applied to synthesize FeNPs. MNNPs were synthesized by thin-film hydration methods and their characteristics such as size and surface morphology were determined by DLS, TEM, respectively. Vibrating sample magnetometer (VSM) was used to evaluate the superparamagnetism of nanoparticles. the structure of the NPs was assessed by FT-IR. Cell viability was examined by MTT in 24, 48, 72 hours. for investigate cell apoptosis and cellular uptake, the treated cells were checked in during 48 hours by FITC-labeled Annexin V/PI flow cytometry. Results :According to obtained results, silibinin loaded in MNNPs has strong dose- and time-dependent cytotoxicity effects on the cancer cell, as HT-29 cell line apoptosis was induced dramatically. The uptake rate of MNNPs by the HT-29 cell line was higher than controls. DL exhibited a high efficiency and DR showed a high potential in acidic pH in cancer cells compared to the neutral condition. The distribution number, surface charge, and chemical structure of MNNPs were proper to escape from the immunity system and confirmed that the silibinin drug was placed in the nisomes. To sum up, the MNNPs as nanocarriers are excellent candidates for the treatment of cancer cells, especially colorectal cancer. Conclution :this study, silibinin was loaded in MNNPs as a new formula to develop its cell apoptosis in targeting drug delivery. To sum up, the findings show that the MNNPs as nanocarriers are excellent candidates for the treatment of cancer cells, especially colorectal cancer.