Effect of Oleuropein on the expression of miR-92b-3p, miR-124-3p and miR-424-5p in KG-1 and HL-60 cell lines treated with 5-Azacitidine

Abstract

Effect of oleuropein on the expression of miR-92b-3p, miR-124-3p and miR-424-5p in human myeloid leukemia KG-1 and HL-60 cells that treated with 5-Azacitidine. Fatemeh Najafi , Saeed Solali Department of Hematology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Introduction : Acute myeloid leukemia is the most common leukemia in adults and every year a large number of patients die due to this disease. Conventional treatments of this disease are usually associated with the risk of relapse and overall toxicity. For this reason, the use of treatments that only target cancer cells and do not harm healthy cells has attracted everyone's attention. MicroRNAs are among the factors that can specifically target certain genes. Specific treatments can be performed by increasing the expression of microRNAs that have anti-cancer effects. On the other hand, the use of flavonoids has been noticed in the treatment of cancer along with the expression of microRNA and has reduced the side effects caused by chemical drugs. In this study, the expression of miR-92b-3p, miR-124-3p, miR-424-5p after treatment with oleuropein and 5-Azacitidine in KG-1 and HL-60 cell lines was investigated and also apoptosis caused by this treatment was investigated and identified. Method : In this study, using MTT test, IC50 for 5-Azacitidine and Oleuropein was determined in KG-1 and HL-60 cell lines. Then the cells were treated with both substances. After 24, 48 and 72 hours of cell treatment, the amount of apoptosis induced in them was measured by using flow cytometry technique. Also, the expression of desired microRNAs was checked by Real Time PCR technique. Result : Treatment of cells of both cell line with both drugs increased the rate of apoptosis. It also increased the expression of all three microRNAs.