Evaluation of level of expression of miR 92, miR 21, miR 103 and miR720 in serum of the CRC patients and the control group

Abstract

Colorectal cancer is one of the most common malignancies worldwide. micro_RNAs are a group of non-coding genes that due to their high stability and small size can be used as biomarkers in the diagnosis of various diseases such as cancer. miR-21, miR-720 and miR-103 miR-92 are important genes whose expression and epigenetic changes have been evaluated and confirmed in several cancers. In this study, the aim of this study was to investigate the relative changes in the expression levels of these genes in serum samples of patients with colon cancer. Method: During this study, serum samples from 50 patients and 50 samples from healthy individuals were collected as a control sample. After collecting the samples, the expression of the desired gene was evaluated using real-time PCR. Differences in expression levels of the two groups were assessed by appropriate statistical tests. In statistical tests, Pvalue <0.05 was considered significant. Result: The expression of all genes studied in this study had significant changes in the patient group compared to the control group. But these changes were not related to any of the clinicopathological features of the patients.