Evaluation of the prophylactic effect of Cromolyn sodium and natural honey of Azerbaijan region on neuropathy caused by Vincristine in mice

Abstract

Introduction: In the process of the formation of neuropathy caused by the administration of anticancer drugs, several mechanisms have been suggested, including activation of the oxidative stress system, increased activity of NMDA receptors and serotonin receptors. Cromolyn-sodium and honey have anti-inflammatory and antioxidant effects.Goal: The purpose of this study was to investigate the preventive effect of Cromolyn-sodium and honey on neuropathic pain caused by Vincristine injection in mice.Materials and methods: 81 mice weighing between 25 and 35 grams were randomly divided into 9 groups of 9 and underwent Cromolyn-sadium and honey regimens for 25-days. Different doses of Cromolyn-sodium and honey were injected three days before injection of Vincristine (1 mg/kg, IP) at fourth day. Next, 3 days of drug injection, 3 days of rest and one day of hot plate test were performed. This 7-days cycle was repeated 3 times in all groups. At the end of the 25th day, blood samples were collected for MDA and TAC estimation. Result: The results indicate a significant increase (p<0.05) in the reaction time to pain in animals at a dose of 12 mg/kg of Cromolyn-sodium and doses of 100,200 mg/kg of honey. Also, a significant effect (p<0.05) of all three doses of Cromolyn-sodium in reducing MDA and a dose of 200 mg/kg honey in increasing TAC was observed, the combined injection of the minimum doses of these drugs, compared to the injection of each of them alone, had an effect It showed statistically significant (p<0.05) increase in TAC and decrease in MDA.Conclusion: Due to inhibition of oxidative stress, Cromolyn-sodium and honey have beneficial effects in preventing neuropathy caused by Vincristine. In order to obtain the exact mechanism of neuropathy prevention, we will need additional studies.