Comparison expression levels of interleukins 10, 1-β and proteins involved in atherosclerosis in the blood of obese and normal weight individuals

Abstract

Atherosclerosis is one of the leading causes of cardiovascular diseases. In this study, the association of serum levels of pro-inflammatory markers interleukin (IL)-1β and IL- 10, C-reactive protein (CRP), cyclooxygenase-2 (COX2), High-density lipoprotein (HDL), Apolipoprotein A1(ApoA1), and ATP Binding Cassette Subfamily A Member 1 (ABCA1) inflammatory proteins with atherosclerosis index (homocysteine) in normal-weight and obese male subjects was assessed. Methods: 59 males including 30 obese (Body mass index (BMI) of ≥ 30 kg/m2) and 29 normal-wight (BMI of 18.5-24.9 kg/m2) were joined to this study. Plasma levels of IL-1β and IL-10 (pg/ml), CRP (pg/ml), COX-2 (ng/ml), APOA1 (mg/dl), ABCA1 (ng/ml), HDL, Cholesterol, and Triglyceride (TG) (mg/dl), and homocysteine (µmol/l) was measured with Real Time PCR. Association of these biomarkers with homocysteine was determined via Spearman and Pearson correlation. Results: Obese subjects had higher serum levels of IL10, IL1β, CRP, COX-2, TG, and cholesterol concentrations (all p<0.05 except IL-10 and cholesterol) and low levels of HDL, APOA1, and ABCA1 (non-significant differences) in comparison to normal-weight group. Homocysteine levels were high in obese men with no significant differences between the two groups. In obese subjects, homocysteine had a significant inverse correlation with APOA1, ABCA1, and HDL, and a strong and moderate positive correlation was found with CRP and TG levels, respectively.