Relationship of Telomerase Activity in Patients undergoing Hemodialysis with parathyroid hormone levels

Abstract

Telomeres are specialized structures located at the ends of eukaryotic chromosomes that are created by replicating small DNA sequences. Telomeres were shortened with each cell proliferation. One of the accelerating factors of telomere shortening is oxidative stress. Secondary hyperparathyroidism is a common disorder in chronic kidney disease that most affects individuals. This choice is made by steadily increasing the level of iPTH. The aim of this study was determination of the relationship between telomerase activity and parathyroid hormone levels in patients undergoing hemodialysis. Method: The telomerase activity of the studied cells was measured based on the Telomeric Repeat Amplification Protocol (TRAP) under a sensitive and special method with an enzyme called PCR-based function. This was done using TeloTAGGG telomerase PCR ELISAPLUS for people with different levels of iPTH. ONE-WAY ANOVA Tukey's test was used to analyze the data. Results: According to the information obtained from this study, increasing the level of iPTH in hemodialysis patients increases the level of telomerase activity, which leads to an increase in telomere shortening speed and cell aging. These changes may be due to oxidative and inflammatory stresses caused by elevated iPTH levels in these patients.