و کورکومین بر تمایز و پرولیفراسیون و چسبندگی سلولهای بنیادی پالپEDTA مقایسه تاثیر هیپوکلریت سدیم و

Abstract

Introduction: This study compared the effects of 1.5% NaOCl, 17%EDTA, and 2.307 ug/ml curcuminsolution on the proliferation, attachment, and differentiation of dental pulp stem cells (DPSCs) on dentin. Methods: MTT assay was used to evaluate the proliferation of DPSCs on dentintreatedwith various concentrations of NaOCl, 17%EDTA, and curcumin (0.97 to 250 µM). Cell-adhering ability andthe LDH assay were used to calculate the number of attached DPSCs. The western blotting assay was performed to measurethe expressionof fibronectin as a cell-adhesion marker and the expression levels of differentiation markersto detect the odontogenic potential of hDPCs: DMP-1, OCN, ALP, and DSPP. Results: The cytotoxicity of irrigants increased with increased dosage, particularly NaOCl. The difference between the cell-adhesion ability of NaOCl and curcumin was not significant (~4.4 MU/mL), whereas EDTA (~3.8 MU/mL) exhibited the lowest release of LDH and least? damage to hDPSCs. However, NaOCl increased fibronectin expression more than EDTA and curcumin. Allthe treated groups upregulated the expression of DSPP, DMP-1, OCN, and ALP compared to the control group, in which NaOCl showed a higher effect on the overexpression of differentiation markers. Conclusion:All the tested irrigants could be used in regenerative endodontic treatment. However, curcumin, an herbal-based and biocompatible irrigant,exhibitedfewer adverse effects than NaOCl and EDTA.