Effect of oleuropein and genistein on apoptosis in HL60 and KG1 cell lines

Abstract

Acute myeloid leukemia is the most common leukemia in adults and every year a large number of patients die due to this disease. Conventional treatments are usually associated with the risk of recurrence and general toxicity. That is why the use of treatments that only target cancer cells and do not harm healthy cells has attracted everyone's attention. Also, due to the side effects of treatment with chemical drugs, today the use of herbal medicines to reduce the side effects of chemical drugs has been considered. Therefore, the use of flavonoids in the treatment of cancer has been considered. In this study, the apoptotic effects of flavonoids oleuropein and genistein in KG-1 and HL-60 categories were investigated and it was found that both factors cause apoptosis. Bcl2 gene expression decreases and Bax gene expression increases. Materials and Methods: In this study, using the MTT test, the IC50 dose for oleuropein and genistein in the KG-1 and HL-60 cell lines was determined. The cells were then treated with both substances. After 12, 24 and 48 hours of cell treatment, the amount of induced apoptosis in them was measured by flow cytometry technique. Also, the expression level of Bcl2, Bcl-xl, Bad and Bax genes was evaluated by Real Time PCR technique.