Evaluation of chemical enhancers on diphenylcyclopropenone topical absorption on rat skin

Abstract

Introduction: Skin is one of the most important tissues of the body that can be affected by autoimmune diseases In comparison to other drug delivery methods, topical drug delivery method has many advantages which may be mentioned as noninvasive and simple way to drug delivery and also it is less toxic than other consuming ways.One of the effective immunotherapy drugs in curing alopecia areata is Diphenylcyclopropenone. . . . . . . . . . . . . . . . . . . . . . . The Objectives: The main objective of this study is to enhance percutaneous absorbtion of drug by chemical enhancers then make comparison between the formulations. Methods: At first, formulations of emulgels were produced by Carbopol 934 and Aerosil 200 as gelling agent, for different ratios of drug components.Then, chemical enhancers added to formulations.Then, the percutaneous absorption of selected formulations through rat skin was examined by Franz diffusion cell device and amount of drug concentration in samples were analyzed by HPLC device in the wavelength of 290 nm.

Results:AUC in percutaneous absorbtion at hours 4 and 1 for formulation that contained Transcutol P were 59.48 and 12.71, and also flux was 11.16.AUC in micro emulgel formulation is 11.01 at 4 hour,and flux is 0.402.

Discussion and Conclusion: Emulgel formulation that contained chemical enhancers specialy Transcutol P significantly enhances percutaneous absorption of Diphenylcyclopropenone compared to simple gel and emulgel formulations.Hence, it goes without saying that emulgel based formulations that contained Transcutol P would be more suitable and reasonable for use in drugs with topical delivery of Diphenylcyclopropenone.