Preventive effect of hydroalcholic extract of grape seed and sodium cellinite on neuropathic pain induced by Vincristine in mice

Abstract

Introduction: In the process of neuropathy induced by anticancer drugs, several mechanisms have been mentioned such as activation of the oxidative stress system, increasing the activity of NMDA receptors, and serotonin receptors. Goal: The aim of the current study was to evaluate the preventive effects of sodium selenite and the grape seed extract on vincristine-induced neuropathic pain that was the result of vincristine in the (spurious) mice. Materials and methods: 80 male mice in a weight range of 25 to 35 grams were randomly divided into equal groups and underwent sodium selenite and the grape seed extract for 18 days. Different doses of sodium selenite and grape seed extract were injected three days before prescription and on the fourth day of vincristine prescription (1 mg/kg,IP ). Then injection of grape seed extract and sodium selenite continued from the fifth up to the ninth day, after vincristine administration. Hot plate test was performed in all groups from first up to the last day and with an interval of three days. Experiments, animals were euthanized and then blood samples were collected for MDA and TAC estimation. Result: The results showed a meaningful increase in reaction to the pain injection of these doses (0.25, 0.5, 1mg/kg, IP) of sodium selenite and grape seed extract (25mg/kg, IP) and also the meaningful impact of sodium selenite in these doses (0.25, 0.5, 1mg/kg, IP) can decrease MDA level. Conclusion: It is probable that a part of the inhibitory effects of sodium selenite is mediated by the control of oxidavtive stress. At the same time, we suggest further studies to obtain precise mechanisms.