Phytochemical investigation of Artemisia marschalliana
چکیده
Introduction: Artemisia marschalliana (Asteraceae) is one of the reported 34 species of genus Artemisia growing in Iran known as ‘Darman-e Arasbaaraani’.
Goals: Due to numerous reported biological effects from genus Artemisia, It is rational to carry out a study on phytochemical analysis of A. marschalliana and subsequently separation and purification of chemical compounds.
Method: Different extracts of this plant (n-hexane, DCM and MeOH), have been obtained using a soxhlet apparatous respectively. Subsequently, the methanolic extract was dried and subjected to a C18 sep-pack using a step gradient of MeOH/water, to get different fractions. Chemical structure of 5 separated compounds were elucidated with 13CNMR and 1HNMR and one with COSY, HSQC, HMBC and DEPT. Essential oil extraction is carried out with water distillation method using a Clevenger device and essence content was determined by GC-MS analysis. Moreover, anti-oxidant and anti-microbial properties of extracts were determined by DPPH reagent and Disc diffusion and MIC methods, correspondingly. In addition, total phenolic and total flavonoid contents were performed as well.
Results: Phytochemical investigation of methanolic extract yielded phenolic, phenylethanoid & benzoic acid derivatives. GC/MS analysis of essential oil led to identification of 38 compounds mostly terpenoids. Methanolic extract and to be more detailed, the 20% and 40% fractions, revealed the most anti-oxidant property and total phenol and total flavonoid contents, respectively.
It is noteworthy that n-hexane extract exibited the most anti-microbial effect.
Conclusion: Phytochemical investigation of methanolic extract led to 5 non-volatile and 38 volatile compounds. Presence of phenolic derivatives seems to be an important feature to show different biological activity.