The effect In vitro of glucosamine on the dialysis rate of beta-blockers

Abstract

One of the most important steps in the bioavailability of drugs is the absorption of the drug from the gastrointestinal tract. Glucosamine is one of the six amino-carbohydrates that are water-soluble and are derived from the hydrolysis of chitosin or chitin. Beta-blockers are beta-adrenergic blocking agents, a class of drugs that bind to the beta-adrenergic receptor and prevent the binding of norepinephrine and epinephrine to this receptor. A review of published papers shows that no studies have been performed to investigate the effect of glucosamine on the absorption of most drugs, including beta-blockers, so in this study, absorption in in vitro model and dialysis rate has been studied. Aim: The aim of this study was to evaluate the effect of glucosamine on the dialysis rate of beta-blockers. Materials and methods: Dialysis is a dialysis cassette made of cellophane or clodion that does not allow colloidal particles and large molecules to pass due to the size of the cassette holes. Svneral concentrations of this glucosamine and the drug were prepared in micrograms per ml and dialysis cassette was used for testing. As such, it is used in the cassette enclosure of the drug and glucosamine solution and then the entire cassette is placed in a chamber that acts as the receptor compartment. Finally, the concentation of the drugs in the samples were determined by HPLC. The dialysis rate was tested triple in the presence of different concentrations of glucosamine. Results: The results showed that glucosamine had a direct effect on the passage of propranolol and atenolol. Conclusion: Our results indicated that glucosamine can be effective in increasing the dylys speed of beta blockers. The optimal ratios of drug to glucose were 1:10 and 1: 5 for propranolol and atenolol, respectively.