Show simple item record

dc.contributor.advisorbabaloo, amirreza
dc.contributor.advisorsandoughchian, siamak
dc.contributor.authorParvan, Sahel
dc.date.accessioned2019-03-10T07:36:08Z
dc.date.available2019-03-10T07:36:08Z
dc.date.issued2019en_US
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/59818
dc.description.abstractIntroduction: The inappropriate inflammatory response is the cause of many common diseases, namely periodontitis. Considering that no studies have been carried out to investigate the effect of IL-36γ on chronic periodontitis, this study aimed to investigate the inflammatory mechanism of IL-36γ by stimulating macrophage cells using NF-KB pathway. Method: This experimental study was performed on 50 healthy individuals and 50 individuals with chronic periodontitis. In this study, macrophage cells were extracted first, and then, RNA was isolated from all samples using TRIzol method. After that, the rate of IL-36γ gene expression was analyzed and compared using real-time PCR technique. Additionally, immunofluorescence (IF) method was used in the current study to investigate the rate of inflammation. The rate of NF-Kb expression was also measured via Western Blot method. Finally, the statistical analysis of samples was done using the appropriate statistical methods in SPSS 17 software. Results: It was found that the rate of IL36γ expression in individuals with periodontitis has increased compared to healthy group (P-value<0.05). Moreover, the obtained results showed that following cells treatment by TLR4, the rate of IL36γ expression increased significantly, and most of it was related to 12 hours after treatment. Discussion and Conclusion: This indicates that after stimulating the TLR pathways, the rate of IL36γ expression will probably increase.en_US
dc.language.isofaen_US
dc.publisherTabriz University of Medical Sciences, School of dentistryen_US
dc.relation.isversionofhttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/59817
dc.subjectChronic periodontitis, Inflammation, IL-1en_US
dc.titleEvaluation of IL36 gamma expression effect on chronic periodontitis by enhancing of NF-KB signaling pathwayen_US
dc.typeThesisen_US
dc.contributor.supervisorshirmohammadi, adileh
dc.identifier.docno603082en_US
dc.identifier.callno59211en_US
dc.description.disciplinedentistryen_US
dc.description.degreedds degreeen_US
dc.citation.reviewerkhoramdel, Azin
dc.citation.reviewerFaramarzi, Masoumeh
dc.citation.reviewerChitsazi, Mohammad Taghi
dc.citation.reviewerSamiee, Mohammad
dc.citation.reviewerBahlpli, Sepideh


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record