Evaluation of IL36 gamma expression effect on chronic periodontitis by enhancing of NF-KB signaling pathway

Abstract

Introduction: The inappropriate inflammatory response is the cause of many common diseases, namely periodontitis. Considering that no studies have been carried out to investigate the effect of IL-36γ on chronic periodontitis, this study aimed to investigate the inflammatory mechanism of IL-36γ by stimulating macrophage cells using NF-KB pathway. Method: This experimental study was performed on 50 healthy individuals and 50 individuals with chronic periodontitis. In this study, macrophage cells were extracted first, and then, RNA was isolated from all samples using TRIzol method. After that, the rate of IL-36γ gene expression was analyzed and compared using real-time PCR technique. Additionally, immunofluorescence (IF) method was used in the current study to investigate the rate of inflammation. The rate of NF-Kb expression was also measured via Western Blot method. Finally, the statistical analysis of samples was done using the appropriate statistical methods in SPSS 17 software. Results: It was found that the rate of IL36γ expression in individuals with periodontitis has increased compared to healthy group (P-value<0.05). Moreover, the obtained results showed that following cells treatment by TLR4, the rate of IL36γ expression increased significantly, and most of it was related to 12 hours after treatment. Discussion and Conclusion: This indicates that after stimulating the TLR pathways, the rate of IL36γ expression will probably increase.