Transformation of E.coli bacteria using PAMAM dendrimers

Abstract

Transformation is as an essential and inseparable part of recombinant in the production of drugs, plants and animals. Nanotechnology advances may lead to the nanoparticles production that has the ability to transfer genes into bacterial cells. In this paper a new method is presented for transformation of E.coli bacteria using PAMAM G2 dendrimer nano polymer. Therefore, PAMAM-DNA complex was prepared by combining the one micrograms of PUC-18 plasmid vector and two micrograms of PAMAM dendrimer polymer. Then, the obtained complex whit bacterial suspension was prepared in 50 mM CaCl2 combined during 7 hours. Also, bacterial suspensions treated with ultrasound were used to facilitate the transfer of PAMAM-DNA complexes. The results showed that PAMAM G2 nanoparticles have the ability to transform bacterial suspension prepared in 50 mM CaCl2. The results showed that cell suspensions treatment with ultrasonication significantly increased the transformation efficiency.