Sequence assigning of the bone morphogenetic protein 15 (BMP15) genes in markhoz goats

Abstract

This research was performed to sequence the bone morphogenetic protein 15 (BMP15) from Transforming Growth Factor beta (TGF?) family in Markhoz breed of goat. This gene controls the ovarian follicle development, ovulation rate and fertility. Blood samples were collected from Sanandaj Markhoz goat breeding station and DNA was extracted using the phenol chloroform method. The primers for exon 1 and 2 were designed and used for amplification of the gene fragments through the simple PCR procedure. The length of exon 1 and 2 are 328bp and 857bp, respectively. Sequence detection was performed after amplification of the gene parts. A mutation at exon 1 in base No.200 with C?T type and two mutations at exon 2 in base No.573 with G?A type and No.755 with T?G type were identified. These mutations were reported and registered in the NCBI gene bank with number of GU732196.