Recognition of the most components in the most effective hydromethanol fraction of Iranian black tea on lipid profile, oxidative stress and inflammatory factors in type I Diabetic rats

Abstract

Objectives: Reliable findings indicate that lipid profile ,oxidative stress and inflammatory factors disorders has the main role in pathogeneses of diabetes and its complications. Traditionally plant extracts have been used for diabetes control. This study was aimed to recognition of the most components in the most effective hydromethanolic fraction of Iranian black orthodox tea on lipid profile, oxidative stress and inflammatory factors in type I Diabetic rats. Methods: This study was conducted on 35 rats which were randomly divided into 5 groups (7 rats in each group). The weight of rats was 200 - 250 gr. Total of groups was diabetic, and diabetes was induced by intraperitoneal injection of streptozotocin 60mg/kg. Groups 1 to 5 were received: carrier, methanol fractions of 20%, 40%, 60%, 80% plus 100% respectively. In the end of study biochemical factors was measured. Data were analyzed by using one ways ANOVA, SPSS statistic methods. Analytical, Preparative and NMR were used to recognize of components in the most effective fraction. Results: In this study concluded that the effective fraction on biochemical factors was 20% .The main component of 20% fraction were caffeine, epicatechingallat, quercetin and kaempferol. Conclusion: It can be concluded that injection of 20% fraction of black tea had positive effect on the prevention of incidence and develop of diabetes complications.