Optimizing the use of Sporosarcina pasteurii bacteria for the stiffening of sand

Abstract

Sporosarcina pasteurii is one of the soil inhabitant and urease-producing microorganisms from which urease enzyme is made, causing bio-calcification. In this research, optimizing of urease enzyme produced by Sporosarcina pasteurii bacteria was targeted to connect the sand particles and stiffen them by the production of calcite precipitation. Bacterium was cultivated aerobically in a culture medium containing yeast extract, lacto pepton and urea at the temperature of 25°C. Different conditions of cultivation with Urea concentration calcium chloride and pH were examined to obtain optimal results. The optimum condition for the stiffening of sand grains was attained in the presence of urea (20 g/L), calcium chloride (30mM) and the pH of 9. The results of this research indicated that the sand calcification via urease enzyme produced by Sporosarcina pasteurii could be employed for the hardening of the sand provided that the efficient factors of calcite precipitation could be optimized. © Global Science Publications.