Anti-oxidative effect of simvastatin in liver and lung tissue after hepatic ischemia/reperfusion in rat

Abstract

In this research we studied simvastatin effect on liver and lung tissue anti-oxidant enzymes, 24 h after hepatic I/R. Male Wistar rats were randomly assigned into three groups of fifteen animals in each group. Group 1, the sham group, underwent laparotomy but did not experience I/R; group 2, the I/R group, underwent ischaemia for 30 min and were reperfused for 24 h; Group 3 underwent ischaemia and reperfusion like group 2 but also received 10 mg kg-1 simvastatin dissolved in water by oral gavage for three days before the operation. In animals subjected to hepatic I/R port triad clamped for 30 min following by 24 h reperfusion. Data showed that GPx and SOD levels in liver and lung tissue in I/R+simvastatin group were higher than I/R group significantly (p<0.05). MDA level in I/R group was significantly higher than I/R+simvastatin group in liver and lung tissue significantly (p<0.05). As simvastatin is a safe drug and result of our previous research and also this research show the efficacy of simvastatin pretreatment in decreasing liver and lung oxidative stress after hepatic I/R, so it may be useful in attenuating hepatic I/R injury and it may be effective for performing safer hepatic surgeries.