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Determination of mefenamic acid in human urine by means of two spectroscopic methods by using cloud point extraction methodology as a tool for treatment of samples

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تاریخ
2006
نویسنده
Tabrizi, AB
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نمایش پرونده کامل آیتم
چکیده
Cloud point extraction was used to extract mefenamic acid (MF) from human urine, and spectrofluorimetry and spectrophotometry were used to analyze extracted ME The variables affecting extraction and phase separation, i.e. HCl and Triton X-114 concentration, temperature and time of equilibration, were optimized. Under the experimental conditions used the limit of detection for extraction of 25 mL of sample was 0.006 and 0.045 mg L-1, with relative standard deviations of 2.52 and 1.45% (n = 5) for spectrofluorimetric or spectrophotometric methods, respectively. Good recoveries in the range of 95-107% were obtained for spiked samples. The proposed methods were applied to the determination of MF in human urine.
URI
http://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/51845
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