Detection limit enhancement of antiarrhythmic drugs in human plasma using capillary electrophoresis with dispersive liquid-liquid microextraction and field-amplified sample stacking method

Abstract

Background: A new capillary zone electrophoresis (CZE) with ultraviolet detection method has been developed and validated for the analysis of four antiarrhythmic drugs in human plasma samples. Methods: In this study, a dispersive liquid-liquid microextraction (DLLME) coupled with field-amplified sample stacking (FASS) was employed for biological samples clean-up and sensitivity enhancement in CZE. Results: Under optimum DLLME-FASS-CZE conditions, enhancement factors were in the range of 157-314. The method was validated over the concentration range of 20-800 ng/ml in human plasma. Inter- and intra-day precision and the accuracy were less than 20%; the detection limits ranged from 2.5 to 4.7 ng/ml. Furthermore, the validated method was successfully applied to the detection of studied drugs in patients' plasma samples.