Targeted superparamagnetic nanoparticles coated with 2-deoxy-D-gloucose and doxorubicin more sensitize breast cancer cells to ionizing radiation

Abstract

Introduction: Nanoparticles are promising as a new approach to enhance chemo-radiotherapy efficiency in breast cancer mainly via targeted therapy. Materials & methods: SKBR3 and T47D breast cancer cells were treated with superparamagnetic mew porous hydroxyapatite nanocomposites (SPmHANs)conjugated with 1 mu M doxorubicin and 0.5 mM 2-Deoxy-o-Glucose and irradiated with 1 and 2 Gy gamma rays in vitro. The treatment toxicity and also the apoptosis/necrosis ratio were measured by MIT assay and also ELISA cell death detection PLUS, respectively. Results: The decreased cell viability with the combined treatment, with determined 42% loading efficiency for 200 ppm 2DG and 93% for5ppm doxorubicin on SPmHANs in PH about 7.4 and 5.5, were calculated to 60.9% and 68% compared to radiotherapy alone inT47D and SKBR3 cells (both with p < 0.05), respectively. Conclusion: Breast cancer cure may boost from The combined targeted nanoparticle treatment with doxorubicin and 2-Deoxy-o-Glucose may boost breast cancer radiotherapy by improved chemodrug localization, increased cytotoxicity in tumor cells and decreased single modality treatment doses. (C) 2017 Elsevier Ltd. All rights reserved.