In Vitro Evaluation of Achillea Millefolium on the Production and Stimulation of Human Skin Fibroblast Cells (HFS-PI-16).

Abstract

In the present study, we aimed the effects of the hydroalcoholic extract of Achillea millefolium (HEAML) on human skin fibroblast cells (HSF-PI-16) proliferation, stimulation and growth properties.Initially, using HSF-PI-16 monolayer culture, we created one line scratch method as an in vitro wound closure and after 3 days monitored via an inverted microscopy.HEAML selectively inhibited proliferation of HSF-PI-16 cells at higher concentration (>20.0 mg/mL), and stimulated at lower concentrations (<20.0 mg/mL). Following, HSF-PI-16 media treatments up to 72 h, HEAML demonstrated significantly elevated proliferation rates (p<0.05) and stimulation in a scratch wound assay (p<0.04). Furthermore, the morphological analysis of HSF-PI-16 cells at culture media were detected the figures of round to spindle, non-adherent, immature and mature cells.These results clearly demonstrate the absence of any toxic effect of HEAML on human skin fibroblasts. To the best of our knowledge, this is the first report elucidating potential mechanisms of action of HEAML on fibroblasts proliferation, and stimulation, offering a greater insight and a better understanding of its effect in future studies.