Effect of different mixing methods on the bacterial leakage of white portland cements and white MTA (Mineral Trioxide Aggregate) when used as root-end filling mTERIALS

Abstract

White Portland Cement and White Mineral Trioxide Aggregateare being used routinely for restoration, root canal obturation, direct pulp capping, furcation perforation repair and apexification.The purpose of this study was to evaluate the effects of different methods of mixing (conventional, amalgamator, ultrasonic) onthe bacterial leakageof MTA and PC used as root-end filling materials Materials and methods: A total of 100 permanent single-rooted human teeth were selected and after removal of soft tissues from root surfaces and sterilization, the teeth were sectioned at CEJ. After root canal preparation, 2 mm from the apicalend of the canalwas cut away. Retrograde cavities were prepared by NSK ultrasonic device and devided into 6 groups( 15 per group) randomly. In addition 10 samples (5 samples in each groups) were considered as positive and negative controls. Mixing of powder and liquid of MTA and PC were carried out in 3 methods: amalgamator, conventional, ultrasonic. The mixies were placed in retrograde cavities. The samples were placed in BHI suspension for evaluating microbial leakage. Description and statistical methods andKaplan-Meier curves and long rank test and SPSS software were used to analyze data. Result: Different mixing techniques showed no imfluence on bacterial leakage of MTA(p=0.224) and PC(p=0.949). There was also no significant difference in bacterial leakage of MTA and PC over 120-day period.(p=0.26) Conclusion: The results showed that various mixing methods did not have any influence on bacterial leakage properties between MTA and PC.