Antibacterial effect of three endodontic sealers(AH 26, Endofill and Biofill) on Enterococcus faecalis in different time intervals : an in vitro study

Abstract

Antimicrobial properties of endodontic sealers would influence the success rate of root canal therapy. Therefore the purpose of this study was to compare the antimicrobial effect of AH 26, Endofill and Biofill on Enterococcus faecalis by Direct contact test (DCT).Materials and Methods : 25 plates were cultured with E. faecalis suspension(1.5 ×10 8CFU/ml) (Colony Forming Unit) on Muller Hinton agar. Four pits were prepared on medium surface by metal punch and the freshly mixed sealers were applied to three of them. The fourth pit was maintained empty as positive control. After incubation (5% Co2 , 37c) , diameter of the bacterial growth-free area around sealers was measured at 3, 5, and 7-day intervals.Results:Statistical analysis revealed that there was a significant difference in growth-inhibition area grade among all experimental sealers grades at all study intervals (p<0.01) Endofill sealer beard more growth-inhibition diameter in comparison to two other sealers (p<0.01).Conclusions:Endofill sealer has more antibacterial effect rather than AH 26 and Biofill on Enterococcus faecalis. The antibacterial effect of sealers did not show any change over time.