بررسی اثر تزریق سلول های C-Kit+ استخراج شده از مغز استخوان بر سطوح پلاسمایی هورمونهای FSH، LH، تستوسترون، التهاب و بیان ژن های مرتبط با آپوپتوز در بافت بیضه موشهای صحرائی نر دیابتی نوع دو
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دانشگاه علوم پزشکی تبریز، دانشکده پزشکی
Tabriz University of Medical Sciences, Faculty of Medicine
Tabriz University of Medical Sciences, Faculty of Medicine
Abstract
دیابت به عنوان یکی از شایع¬ترین اختلالات متابولیک دارای عوارض متعددی بر سیستم¬های بدن می¬باشد. یکی از عوارض قابل¬توجه دیابت در مردان اختلالات در سیستم تولیدمثلی است که نیازمند مداخلات درمانی است، بنابراین در مطالعه حاضر اثرات سلول¬های C-Kit+ و C-Kit- بر اختلالات تولید مثلی ناشی از دیابت شامل تغییرات هورمون¬های جنسی، فاکتورهای التهابی و میزان آپوپتوز در رت¬های دیابتی نوع2 را مورد بررسی قرار دادیم
مواد و روش¬ها: در این مطالعه تعداد 48 سر موش صحرایی نر استفاده شد. تعداد 12 سر از این حیوانات در گروه کنترل قرار گرفتند که رژیم غذایی نرمال را دریافت کردند. در حالی¬که تعداد 36 رت دیگر به مدت 4 هفته رژیم غذایی پرچرب دریافت کردند و بعداز پایان 4 هفته، تک¬دوز استرپتوزوتوسین (STZ) برای القاء دیابت بصورت داخل صفاقی تزریق شد. رت¬هایی را که دیابت با موفقیت در آن¬ها القا شد به طور تصادفی در 3 گروه شامل، دیابتی درمان نشده، دیابتی تیمار شده با سلول های C-Kit- و دیابتی درمان شده با C-Kit+ تقسیم شدند. پنج روز بعد از تزریق STZ و بدنبال تائید دیابتی شدن تزریق سلول¬های C-Kit و یا حلال انجام شد. بعد از آن حیوانات به مدت 60 روز نگهداری شدند و در این مدت تمام گروه¬ها رژیم غذایی نرمال دریافت کردند. بعد از پایان این مدت، حیوانات پس از بیهوشی عمیق آسان کشی شده، مقادیر هورمون¬های FSH، LH و تستسترون در سرم، و همچنین میزان فاکتورهای IL-1β، NF-kB، BCL-2 و BAX در بافت بیضه اندازه¬گیری شد.
یافتهها: دیابت باعث کاهش هورمون¬های FSH، LH و تستسترون، فاکتور IL-1β و بیان پروتئین BCL-2 (p<0.01 تا p<0.01) شد، درحالی که میزان NF-kB (p<0.001) و بیان پروتئین BAX (p<0.01) را افزایش داد. تیمارهای اعمال شده تا حدودی با این تغییرات ناشی از دیابت مقابله کردند.
Diabetes, as one of the most common metabolic disorders, has numerous complications on body systems. One of the significant complications of diabetes in men is reproductive system problems that require therapeutic interventions. Therefore, in the present study, the effects of C-Kit+ and C-Kit- cells on diabetes-induced reproductive disorders, including changes in sex hormones, inflammatory factors, and apoptosis levels in type 2 diabetic rats, were investigated. Method and Materials: In this study, 48 male Wistar rats were used. 12 of these animals were assigned to the control group and received a normal diet. The remaining 36 rats received a high-fat diet for 4 weeks. At the end of 4 week, a single dose of streptozotocin (STZ) was injected intraperitoneally to induce diabetes. Rats in which diabetes was successfully induced were randomly divided into three groups: untreated diabetic, diabetic treated with C-Kit- cells, and diabetic treated with C-Kit+ cells. Five days after STZ injection and following confirmation of diabetes induction, injections of C-Kit cells or solvent were performed. Thereafter, all animals were maintained for 60 days, during which time all groups received a normal diet. After this period, the animals were euthanized and serum levels of FSH, LH, and testosterone hormones, as well as levels of IL-1β and NF-kB factors in testicular tissue were measured. Findings: Diabetes led to a decrease in FSH, LH and testosterone hormones, IL-1β level, and BCL-2 protein expression (p<0.01 to p<0.001), while increased NF-kB level (p<0.001) and BAX protein expression (p<0.01). The applied treatments partially counteracted these diabetes-induced changes.
Diabetes, as one of the most common metabolic disorders, has numerous complications on body systems. One of the significant complications of diabetes in men is reproductive system problems that require therapeutic interventions. Therefore, in the present study, the effects of C-Kit+ and C-Kit- cells on diabetes-induced reproductive disorders, including changes in sex hormones, inflammatory factors, and apoptosis levels in type 2 diabetic rats, were investigated. Method and Materials: In this study, 48 male Wistar rats were used. 12 of these animals were assigned to the control group and received a normal diet. The remaining 36 rats received a high-fat diet for 4 weeks. At the end of 4 week, a single dose of streptozotocin (STZ) was injected intraperitoneally to induce diabetes. Rats in which diabetes was successfully induced were randomly divided into three groups: untreated diabetic, diabetic treated with C-Kit- cells, and diabetic treated with C-Kit+ cells. Five days after STZ injection and following confirmation of diabetes induction, injections of C-Kit cells or solvent were performed. Thereafter, all animals were maintained for 60 days, during which time all groups received a normal diet. After this period, the animals were euthanized and serum levels of FSH, LH, and testosterone hormones, as well as levels of IL-1β and NF-kB factors in testicular tissue were measured. Findings: Diabetes led to a decrease in FSH, LH and testosterone hormones, IL-1β level, and BCL-2 protein expression (p<0.01 to p<0.001), while increased NF-kB level (p<0.001) and BAX protein expression (p<0.01). The applied treatments partially counteracted these diabetes-induced changes.