School of Pharmacy

Permanent URI for this communityhttps://dspace.tbzmed.ac.ir/handle/123456789/5

The School of Pharmacy, as the second pharmacy faculty in Iran after Tehran pharmacy school in 1328 Hejri Shamsi , was located at Tabriz Imam Khomeyni square and Ayatollah Taleghani Street ,was established to provide the country needs to pharmacist. This major change occurs when there was no pharmacy school in Tabriz and pharmacy personnels learn it experimently and finally reach the stage of dispensering . Permission to establish a pharmacy was given after passing the test which requires the literacy and knowing Latin language and dispenesring. Tabriz pharmacy faculty is established after student advertising admission. At the first reception, the number of students did not pass, But college starts first training cources with 25 students. After 2-year curriculum of courses, the speciality pharmaceutical topics were teached. Toxicology and jalinoosi products were as specialized courses. Later by the dean Department , Dear doctor Ismaiil Anghajy , basic steps were taken for developing the faculty. School of Pharmacy began its own activities with medicine school and gained independence in 1345.following the dissolution of the pharmacy schools in whole country in September 1347, a new school of pharmaceutical and clinical laboratory sciences began its own activities.The pharmacy faculty was established with the aim of training pharmacy students and drug experts to manage pharmacy, Pharmaceutical industry,economy of country and training experts to work in clinical laboratory and searching in pharmacy and the laboratory. The Department of Laboratory science was transferred Medicine faculty in 1351. The Nutrition school within Tabriz Pharmacy Faculty began its academic activities with 39 students in 1353 and with the establishment of the health and nutrition school it was separated from pharmacy faculty in 1370. The new building for the School of Pharmacy started with an area of 17,000 square meters in 1386. Tabriz School of Pharmacy has more than 3000 pharmacy graduated is delivered to our country that many of them , have been efficient and effective rules in Pharmaceutical Science. currently Tabriz School of Pharmacy consists of 7 Department of Pharmaceutic, Pharmacology and Toxicology, Pharmacognosy, Pharmaceutical Chemistry, Pharmaceutical Biotechnology, Clinical Pharmacy and Food and Drug Control. Faculty Education departments and physical facilities with high knowledge of professors tries to through training programs, basic and applied researches and provide services , solve problems with use of a pharmacy modern knowledge and Significant contribution in scientific development of their own country. Training courses for PhD students starts in field of Pharmacognosy in Tabriz school of pharmacy . now 62 PhD students are studing in Tabriz Pharmacy faculty in the field of Pharmacognosy, Pharmaceutics, Pharmacology, Pharmaceutical Chemistry, Toxicology, Biotechnology, pharmaceutical and food and drug control. Tabriz Pharmacy school is located at the heart of the city where the science and art were born. preserved old town in the vicinity of modern development, its proximity to the Shahand mountain that always carrying snow and Uromiye lake and ... causes the city unforgettable for students who live and study in it.

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3D structure predictive performance of de novo modeling based on molecular dynamics simulation for polypeptides with experimentally solved structures
(Tabriz University of Medical Sciences , School of Pharmacy, 2023) Karimzadeh Halimi, Nazanin; Hamzeh Mivehroud, Maryam; Sokouti, Babak
Introduction: Proteins are made up of building blocks called amino acids. The wide variety of proteins' functions, these vital constituents of living cells, is due to their specific three-dimensional structure originating from the appropriate folding of amino acid sequence. Protein structure determination has long been of great interest and challenge in the field of molecular design. Aims: In the current study, de novo modeling was employed to assess its predictive performance for structurally-solved polypeptides . Materials and methods: Polypeptides with 20 to 150 amino acids were extracted from a protein data bank (PDB) and their amino acid sequences were retrieved. In the next step, molecular dynamics (MD) simulation was performed for 50 ns in two environments including vacuum and solvated systems. Following the MD simulation, the stability of the whole system in terms of energy and conformational changes was investigated. Then, conformational clustering was conducted, and the structures with the lowest root-mean-square deviation (RMSD) to the centroids of the highly-populated clusters were selected. Moreover, the models with the lowest energy during the simulation time were opted. Then, the secondary structure contents were assigned for the obtained models using DSSP (defined secondary structure of proteins), PCASSO (protein c-alpha secondary structure output), and STRIDE (structural identification) algorithms. Results: Analyses revealed no meaningful relationship between the secondary structure elements of MD- and X-ray based models. The current MD-based de novo method, predicts the three-dimensional (3D) structures for the given polypeptides differently than experimental methodologies. Conclusion: The current study can provide a better insight into the capability and limitations of de novo molecular modeling of proteins.
Acetylcholinesterase inhibitory activity of extract and essential oil of aerial parts of Melilotus officinalis
(Tabriz University of Medical Sciences , School of Pharmacy, 2023) Jafarzadeh, Helma; Khodaei, Laleh; Soltani, Somaieh
Introduction: Alzheimer's is one of the most common neurodegenerative diseases and the most important cause of dementia. Acetylcholinesterase inhibitors have been the most effective drug class in improving the symptoms of this disease. The increasing desire of people to consume natural ingredients has also led our study to this field by citing traditional medicine sources. Melilotus officinalis is a biennial plant with a maximum height of 1 meter native to Iran. In traditional medicine, the flowering branch has been used in inflammatory diseases, insomnia, high blood pressure, anticoagulation, and wound healing. This study aimed to investigate new natural compounds with inhibitory effect on acetylcholinesterase, from the essential oil and extract of this plant were studied.Aim: Acetylcholinesterase inhibitory activity of extracts and essential oil of aerial parts of Melilotus officinalis.Method: The flowering branches of this plant were collected and identified. The essential oil was obtained by Clevenger apparatus and the extracts were acquired by soxhlet extraction. Acetylcholinesterase inhibition of essential oil and extracts was measured by Ellman's method. Acetylthiocholine iodide (ATCI) was used as a substrate, and donepezil was used as a positive control to measure the inhibition of acetylcholinesterase. Molecular modeling studies were performed with AutoDocktools-1.5.6 software to study the interaction mechanism of compounds.Result: In the Ellman test, the highest inhibition was related to freeze-dried ethanolic extract with inhibitory activity of 70.9%, vaccium-dried ethanolic extract with 49.5% inhibition, and freeze-dried methanolic extract with 28.1% inhibition.In docking studies, triterpenoid and flavonoid compounds showed strong interactions in the Enzyme active site.Conclusion: It seems that flavonoid and triterpenoid compounds are among the main effective compounds in the occurrence of cholinesterase inhibitory effect.
Acetylcholinesterase inhibitory activity of extract and essential oil of Heracleum persicum
(Tabriz University of Medical Sciences , School of Pharmacy, 2023) Helali, Maryam; Soltani, Somaieh; khodaei, Laleh
Introduction: According to the signals in traditional medicine sources, the flower of the plant "Golpar" was known for its anti-forgetting property. In the present study, we investigate the effect of this plant on Alzheimer's disease. Heracleum persicum, commonly known as Persian Hogweed. It belongs to the Apiaceae family. Heracleum persicum, in Iranian traditional medicine, is used for the treatment of various diseases including neurological, gastrointestinal, respiratory, rheumatological, urinary tract, and to alleviate stomach bloating.Aim: Investigating the inhibitory effect of Heracleum persicum fruits on acetylcholinesterase enzyme.Methods: The fruit powder of the plant was extracted using steam distillation method to obtain the essential oil, and the Soxhlet apparatus was used to extract the plant's essence. In the first stage, the extractions were performed using petroleum ether, dichloromethane, and methanol solvents, and in the second stage, n-hexane, ethyl acetate, and ethanol solvents were used. The obtained extracts were dried by two methods: vacuum drying and freeze drying. Various concentrations of extracts and essential oils were prepared. Acetylcholinesterase enzyme was extracted from healthy human blood, and the intensity of absorption of different concentrations of the extracts was measured using the Ellman method. The composition of the essential oil was identified using GC-MS equipment. The chemical compounds of the effective extracts were also collected from previous studies. Finally, Molecular modeling studies were performed with AutoDocktools-1.5.6 software to study the interaction mechanism of compounds. Results: According to the results of the Ellman test, the petroleum ether, ethanol, and methanol extracts of the Golpar plants and essential oil have anti-cholinesterase effects. Molecular docking simulation results showed that the identified compounds have good affinity for inhibiting the enzyme.Discussion: It seems that the compounds in the essential oil and extracts of Heracleum persicum have an inhibitory effect on the enzyme acetylcholinesterase.
Acute Anti-Inflammatory Effects of Neostigmine in the Rat Air Pouch Model of Inflammation
(Tabriz University of Medical Sciences, Faculty of Pharmacy, 2018) Haj Hasan, Iman; Najafi, Moslem; Eteraf-Oskouei, Tahereh
Background: Inflammation plays a major role in the pathogenesis of multiple diseases. Because the common anti-inflammatory drugs have many complications. Scientists try to find new methods and drugs to reduce inflammation, which have the least side effects. Aims: The nervous system and immune system have a bidirectional communication. The aim of this study is to investigate the effects of neostigmine on the inflammatory parameters in the rat air pouch model of acute inflammation in male rats. Method: Male Wistar rates were anesthetized, 20 ml and 10 ml of sterile air were injected subcutaneously on the back of animals on day 1 and 3 respectively. On day 6, inflammation was induced by injection of 2 ml of carrageenan 1% into pouch. One milliliter of neostigmine (35, 70, 140, 200 and 310) µg/kg in the test groups and saline in the control group were administered intra peritoneal at the same time with the carrageenan injection. After six hours animals were sacrificed. The pouches were flushed with Phosphate Buffered Saline (PBS). Then, they were opened, pouches fluid was collected in order to determine exudates volume and cell accumulation. The granuloma tissue was extracted and weighed, and its histopathologic characteristics were examined. Results. Exudates volume and tissue weight were only decreased by neostigmine 70 µg/kg significantly (p ≤ 0.001). Leukocytes accumulation also was decreased by neostigmine 70 µg/kg significantly (P < 0.05). The granulation tissue weight was increased significantly by neostigmine 200 µg/kg (p < 0.01). Conclusion: From this study it may be concluded that neostigmine affects on the inflammatory parameters possibly through the modulatory effects on immune system.
Acute Anti-Inflammatory Effects of Salbutamol in the Rat Air Pouch Model of Inflammation
(Tabriz University of Medical Sciences, Faculty of Pharmacy, 2017) Maghsoudi, Milad; Eteraf Oskouei, Tahereh; Najafi, Moslem
Background: Recent studies have proven that immune and nervous systems have a bidirectional communication. Anti-inflammatory effects of β2 adrenergic receptors have been considered in recent years. Aims: The aim of this study is to investigate the effects of salbutamol on the inflammatory parameters in the rat air pouch model of inflammation. Method: Male Wistar rats were anesthetized; 20ml and 10ml of sterile air were injected subcutaneously on the back of animals on day 1 and 3 respectively. On day 6, inflammation was induced by injection of 1ml of carrageenan 1% into pouch. One milliliter of salbutamol (125, 250 & 500 µg/pouch) and salbutamol plus propranolol (500µg/pouch) in the test groups and saline in the control group were administered intra pouch at the same time as the carrageenan. After 6 hours animals were sacrificed. The pouches were flushed with PBS. Then they were opened, pouches fluid was collected in order to determine exudates volume, cells accumulation and IL-1β concentration. The granulation tissues formed were dissected out and the weight determined. Results: Leukocytes accumulation and exudates volume were decreased significantly by salbutamol 250 µg/pouch (P<0.01). The dose of 500 µg/pouch of salbutamol reduced significantly the number of leukocytes (P=0), exudates volume (P=0), granulation tissue weight (P<0.001) and IL-1β level (P<0.01). In addition there were no changes in the inflammatory parameters by co-administration of salbutamol and propranolol. Conclusion: From this study it may be concluded that salbutamol possesses anti-inflammatory property possibly through the stimulation of β-2 adrenergic receptors. This anti-inflammatory effect may be as important mechanism in asthma treatment, where inflammation also has role in the etiopathology.
Affect of Achillea millefolium in Prophylaxis of oral mucositis induced by chemotherapy in AML patients
(Tabriz University of Medical Sciences, Faculty of Pharmacy, 2016) Hajisalem, Telka; Shokri, Javad; Fathi Azad, Fatemeh; Mashayekhi, Simin Ozar; Nejati, Babak
Introduction: Oral mucositis is a common complication of chemotherapy. Various methods and medications have been using to treat oral mucositis. Achillea millefolium L. has anti-inflammatory and antimicrobial effects. Aim: The aim of this study is evaluation of the Achillea millefolium mouthwash effect in prophylaxis and treatment of oral mucositis induced by chemotherapy in patients with Acute Myeloid Leukemia (AML). Materials and methods: In this randomized clinical trial, 30 patients with AML, who received 3+7 or 5+2 regimen, were randomly assigned to intervention (n=15) and control (n=15) groups. The intervention group gargled 10 ml Achillea millefolium mouthwash and also 15 ml Chlorhexidine mouthwash 2 times a day for 20 days from the first day of chemotherapy while the control group gargled 15 mL of Chlorhexidine mouthwash 2 times a day. Oral mucositis and its complications were assessed at the beginning of the intervention, 5, 10, 15 and 20 days of the study. Results: Intervention group had better mean oral mucositis score compared to control group which was significant in day 20 (P value = 0.002). Intervention group had shown decrease trend in both pain occurrence and painkiller consumption. In day 20 fewer patients had pain, and painkiller consumption was lower in intervention group in compare with control group (P value = 0.002, 0.001, respectively). Oral mucositis score had shown development during treatment but in days 10th and 20th results had shown significant different between intervention and control group (P value = 0.021, 0.002, respectively). In comparison of sever and mild mucositis, results had shown that intervention group was significantly better than control group (P value = 0.018). Conclusion: In conclusion, Achillea millefolium extract improved oral mucositis better than chlorhexidine. Although Achillea mouthwash was not effective in prophylaxis of mucositis but it had shown better effects on pain and oral mucositis.
Alzheimer related Target prediction for Glatiramer acetate
(Tabriz University of Medical Sciences , School of Pharmacy, 2023) Majidzadeh, Samin; Soltani, Somaieh; Wolber, Gerhard
Introduction: According to the evidence in previous studies, it was found that GA, which is effective in the treatment of MS, has also shown positive effects on the cognitive effects of AD. Objective: Alzheimer-related Target prediction for Glatiramer acetate.Methods: Using the developed python models and the molecules in other databases, we select the desired target molecules with the Baysian finger print method, which is a method based on similarity.Then we calculate the similarity index of GA with the inhibitors and ligands of the targets in the ChEMBL25 database according to the classification method and select targets with an index greater than 0.9. We rank the selected targets based on experimental evidence and select targets with a higher rank to investigate the molecular mechanism.In order to predict the mechanism of interaction between GA and targets predicted by molecular docking method, we use Autodock software.We validate our calculation method in order to check the correctness of the data by an external method. Results: 107 targets were extracted from the KEGG database and scientific texts, and after using the developed Python model, 2 targets with proba > 0.9 named AMPA1 and GCPⅡ were selected to investigate the interaction and binding mechanism with GA.Conclusion: Using the developed models in this study, effective targets in Alzheimer's disease were identified on the drug glatiramer acetate. The data mining method used made it possible to conduct studies with a large number of targets in a short period. The results of this method can be used to predict the target and find the leading compound.
Analysis Methanolic Extracted Compounds from Vitex Pseudo negundo
(Tabriz University of Medical Sciences, Faculty of Pharmacy, 2018) Sarikhani Khorrami, Mohsen; Asnaashari, Solmaz; Nazemeieh, Hossein; Asgharian, Parina
Introduction: The genus Vitex from Lamiaceae family comprises 250 species in world. Some of the biological activities of the species of this genus such as treatment of female hormone disorders and anti-inflammatory were attributed to the presence of flavonoids and iridoids compounds. Although new chemical drugs have highly effective and very useful for the body, but have countless side effects, this has led the medical community to turn into a herbal remedy for treating a number of diseases. Scope: This investigation was performed in order to analyse the chemical composition of Vitex pseudo negundo and evaluation its antioxidant properties. Methods and materials: Seeds of Vitex pseudo negundo were successively extracted using petroleum ether, chloroform and methanol solvents by soxhlet apparatus. Dried methanolic extract was subjected to C18 sep-pak fractionation using a step gradient of methanol-water Further purification of fractions by using preparative reversed phase HPLC yielded 2 compounds. Structure elucidation of these compounds was accomplished by using 1HNMR and 13CNMR methods. The essential oil was obtained by hydro-distillation using a Clevenger apparatus. The composition of essential oil was determined by GC_MS. Free radical scavenging activity contents of extracts were determined using DPPH radicals. Results: Phytochemical study of the methanolic extracted yielded to identification two compounds. The study on the composition of essential oil yielded 35 compounds which were mainly composed terpenoids.The methanolic extract of herbal showed moderate free radical scavenging activity. Conclusion: Overally, biological activities of this herb might be due to the presence of phenolic derivatives in the methanolic extract.
Analysis of deferiprone in exhaled breath condensate using silver nanoparticle-enhanced terbium luminescence
(Tabriz University of Medical Sciences, Faculty of Pharmacy, 2018) Mohamadian, Esmail; Pharmaceutical Chemistry; Jouyban, Abolghasem
Introduction: In recent years, metal nanoparticles with their specific properties have attracted particular attention due to their improved spectral characteristics. In this study, the effect of the interaction between AgNPs (silver nanoparticles) and the drug– terbium complexes in solution on luminescence intensity was studied, and a significant enhancement of terbium-sensitized luminescence on florescence intensity was observed in the presence of AgNPs. Exhaled breath condensate (EBC) is a biological sample that can provide a direct assessment of pulmonary pathobiology, and its sampling procedure has been shown to be an alternative specimen for drug monitoring. Aim: Study of AgNPs effect on the fluorescence emission of terbium ion– Deferiprone (Tb3+–DFP) in EBC samples and validation of the proposed method under optimal conditions for analysis of DFP (Deferiprone) concentrations in the EBC samples obtained from the patients receiving DFP. Method: To reach the maximum fluorescence intensity, the effects of experimental parameters i.e. pH, buffer concentration, Tb3+(terbium ion) concentration, reaction time, temperature, and AgNP concentration will be investigated. After method optimazation and validation, DFP concentrations in the EBC samples will be measured. Results: Effects of various parameters such as AgNPs and Tb3+ concentrations, pH, and buffer concentration were studied. In the optimum condition, a calibration curve was obtained with a coefficient of determination (R2) of 0.999, and linearity in the concentration range of 0.04–1.50 mg L_1. The method was successfully applied for the quantification of DFP in EBC of 15 patients with very low concentrations of DFP (0.06–0.17 mg L_1). Discussion: In this study, a sensitive method based on AgNPs was developed for the quantification of DFP in EBC samples.
Analysis of the situation of formulation of strategic planning, challenges, and performance strategies based on strategic planning in Tabriz pharmaceutical companies -mixed method (quantitative-qualitative)
(Tabriz University of Medical Science, School of pharmacy, 2021) Hejazi, Seyed mahdi; Hazerati, Hakime; Kiafar, Farhad; Mohammadreza, Siahi shadbad; Hanaee, Jalal
Introduction:With strategic thinking, organizations take the initiative and instead of reacting to actions; They determine their own destiny and take control of the future. Strategic perspective leads to increasing companies' awareness of external threats,better understanding of competing company strategies, and overcoming external threats by being aware of their capabilities and strengths. Aim: The aim of this study is to analyze the situation of how to develop strategic planning, challenges and performance strategies based on strategic planning in Tabriz pharmaceutical companies. Materials and Methods: In a qualitative approach study through semi-structured interviews in a focused group discussion, the experiences of mid level managers of pharmaceutical companies regarding the status of strategic planning, challenges and performance strategies based on strategic planning were examined. Participants in the study were mid level managers of Zahravi and Dana and Shahid Ghazi pharmaceutica factories, and sampling continued until data saturation. A focus group discussion was held with 14 managers of these factories. Data were analyzed using schematic content analysis. Results: From the analysis of interviews, 320 open codes were extracted in three general categories as well as 11 subcategories was extracted. Discussion and Conclusion: Based on the analysis of managers' experiences, it was found that the main factor in the success or failure of the strategic plan are senior managers, so it is necessary for managers to be aware of the strategic plan and understand its importance.
Anti-aging effect of barley stem cell extraction loaded nanoliposome on facial skin
(Tabriz University of Medical Sciences , School of Pharmacy, 2023) Kamali, Mohammad Javad; Kosari, Morteza; Azimi Alamdar, Hamideh; Barzegar Jalali, Mohammad; Hamishehkar, Hamed
Introduction: Agents such as sun radiation may cause skin aging and lyperpigmentation. Using plants in cosmetic treatments is popular as they have fewer side effects. Utilizing plant stem cells is a new approach to formulating cosmetic creams that are plant-based. Plant stem cells are rich in antioxidants, growth factors, and many other compounds, therefore they are an invaluable resource of plant-based active ingredients. Barley has had various applications since a long time ago because of its many different properties recent studies have shown the anti-melanogenesis and whitening effects of the barley plant.Goal: We aimed to Investigating the anti-aging effects of extract from barley plant stem cell culture loaded in nanoliposome on human skin.Methods: Nano-liposomes were prepared by a thin layer method and characterized for size, Polydispersity Index (PDI), zeta potential (ZP),SEM, entrapment efficiency (%EE) and loading capacity (%LC). The in-vivo experiments on 12 volunteers were conducted with the barley extraction on nano-liposome on the right side of the face around forehead and the solution form of extraction on the other side of the face symmetrically, and the effect of these two formulations on reducing wrinkles around the forehead were compared.Results: The developed Nano-liposomes showed proper characteristics for dermal delivery of barley callus extract such as mean particle sizes around 120nm and EE and LC values of %86.9 and %17.56 respectively. In-vivo studies in humans showed the superiority against the controls in the collagen generation in the skin and skin surface smoothness.Conclusion: It was concluded that the nanoliposome formulation containing barley callus extract can be introduced for local administration of barley callus extract in the prevention or treatment of photoaging.
Anti-diabetic effects of Eryngium billardieri on streptozotocin-induced diabetic male mice
(Tabriz University of Medical Sciences, Faculty of Pharmacy, 2020) touri, Elaheh; Gorjani, Alireza; Asgharian, Parnia; Vaez, Haleh
Introduction: The genus Eryngium, with 274 species, is one of the largest genera of the Apiaceae family. Nine species of this genus grow in Iran, and Eryngium billardieri is one of them. E.billardieri has shown numerous pharmacological effects including anti-inflammatory, antimicrobial and antioxidant. Objective: To investigate the anti-diabetic effects of E.billardieri on streptozotocin-induced diabetic male mice. Materials and Methods: Extraction was performed using ethanol 70% by maceration method. Mice were randomly divided into 8 groups (n=6): Healthy control group, diabetic control group, healthy control group of the extract (300 mg/kg Oral), diabetic mice receiving the extract (100 and 300 mg/kg, Oral and IP), group receiving simultaneous STZ (IP) and high dose of extract (Oral) for 5 days. Type 2 diabetes induced by a single dose of STZ (150 mg/kg). Diabetic mice received low and high doses of the extract (Oral and IP) for 18 days. Finally, mice were anesthetized by IP injection of ketamine and xylazine. Blood samples were collected from the heart and serum samples were separated and kept in -70 ° C. Three days before surgery, the OGTT test was performed. Results: IP injection of the extract (300 mg/kg) reduced the weight of diabetic mice and significantly reduced glucose tolerance. Also, gavage of the extract (300 mg/kg) significantly increased serum insulin level, significantly decreased blood glucose, significantly reduced glucose tolerance and MDA. Conclusion: Oral administration of the hydro-alcoholic extract of E.billardieri had antidiabetic activity. The generated data supports the folkloric claims associating E.billardieri with hypoglycemic effects. However, there is need for further studies on this plant to investigate the mechanism of its activity and determine its safety profiles in order to explore possibilities of developing a new antidiabetic drug.
anti-diabetic effects of Marrubium propinquum methanol extract on Streptozotocin -induced diabetic male mice
(Tabriz University of Medical Sciences, School of Pharmacy, 2021) Amirsabokbar, Meysam; Vaez, Haleh; Hamedyazdan, Sanaz
Lamiaceae family has held its therapeutic position in herbal medicine by several known healing attributes. In traditional medicine of several nations, Marrubium plant is used to treat diabetes. In traditional Iranian medicine, Marrubium is prescribed as a tonic for mines, cardiotonic, anti fever, and in foreign applications it is used as a disinfectant. Aim: Evaluation of anti-diabetic effects of methanolic extract of Marrubium propinquum on streptozotocin-induced male diabetic mice Materials and Methods: Methanolic extract of the plant was extracted by Soxhlet method. Male mice were divided into 5 groups. The first group was a healthy control group; the second group (diabetic control group); the third group (a healthy control group receiving the extract at a dose of 100 mg / kg); The fourth group (diabetic group receiving the extract at a dose of 100 mg / kg) and the fifth group (diabetic group receiving the extract at a dose of 300 mg / kg). In order to induce diabetes in rats, 40 mg / kg streptozotocin was injected intraperitoneally to the animal 5 days after dissolution in normal saline. After confirming that the animals were diabetic, the extract was injected daily for 18 days. Before induction of diabetes and then every three days, weight and blood sugar of the animals were measured and two days before surgery, OGTT test was performed. Results: IP injection of 300 mg/kg methanolic extract of M. propinquum reduced blood sugar in diabetic rats and improved oral glucose tolerance; and reduced serum SGOT and SGPT, which were significant for doses of 100 and 300 mg / kg. Conclusion: M. propinquum methanolic extract may play a protective and therapeutic role in diabetes by increasing insulin secretion and decreasing blood sugar and MDA levels and serum SGOT and SGPT enzymes.
Antibacterial activity and preservative effect of phloretin as an alternative for sodium nitrite
(Tabriz University of Medical Sciences, School of Pharmacy, 2024) Moradpour, Farhad; Afshar Moghadam, Mohamadreza; HallajzeZhadi, Somayeh; Siahi shadbad, Mohammadreza; Farjami, Afsaneh
Introduction: Food preservation is a crucial challenge in the food industry to increase shelf-life and ensure food safety. This study investigated the antimicrobial and antioxidant effects of phloretin and its combination with sodium nitrite as a potential food preservative system. phloretin is a natural phenolic compound with reported antimicrobial and antioxidant properties, and sodium nitrite is a common synthetic preservative.Aim: This study aims to explore the potential of phloretin as a substitute for sodium nitrite in meat products. By assessing its antibacterial effects on common meat-contaminating bacteria like Salmonella typhimurium and Escherichia coli, measure lipid peroxidation levels, and quantify biogenic amines in the meat.Methods: The antimicrobial activity of phloretin, sodium nitrite, and their combination against major foodborne pathogens including Salmonella Typhimurium and Escherichia coli, as well as total bacterial count, was evaluated. The antioxidant capacity was assessed through in vitro assays measuring free radical scavenging and lipid peroxidation inhibition. The ability of these compounds to inhibit the production of biogenic amines histamine, tryptamine, and cadaverine as food spoilage markers was also investigated.Results: The combination of phloretin and sodium nitrite showed superior preservative effects compared to either compound alone. This combination more effectively reduced bacterial counts in the tested pathogens, inhibited oxidative stress through improved antioxidant activity, and suppressed cadaverine production to a greater extent. Sodium nitrite alone also exhibited significant antimicrobial and antioxidant properties and in some cases, such as Salmonella inhibition and radical scavenging, outperformed the combination with phloretin. Conclusion: The synergistic preservative effects of the phloretin -sodium nitrite combination suggest that this natural-synthetic blend can serve as an effective strategy to enhance food safety, increase shelf-life, and reduce reliance on individual synthetic additives. Further development and validation of this preservative system can contribute to the advancement of clean-label and sustainable food preservation technologies
Anticarcinogenic effects of methanolic extract of chrozophora tinctoria on Breast cancer cell line MCF-7
(Tabriz University of Medical Sciences, Faculty of Pharmacy, 2018) Abdoli, Ameneh; Dastmalchi, Siavash; delazar, Abbas; Rezazadeh, Hassan; Mohajjel Nayebi, Ali Reza
Introduction: Chrozophora tinctoria is a species from Euphorbiaceae family, naturally occurring annual plant, used as a dyeing substance and traditionally it is used for the treatment of warts and leprosy. In this study we attempt to evaluate the cytotoxic effects of chrozophora tinctoria on breast cancer MCF-7 cell line and comparing with standard chemotherapy agent, including cisplatin using MTT assay. Aim: The aim of this study was to investigate the anticarcinogenic effects of methanolic extract of chrozophora tinctoria on breast cancer cell line MCF-7. Methods: Chrozophora tinctoria were collected and the methanolic extract of the plant were prepared. The cytotoxic effects of extracts were assessed on MCF-7 and HuGu cell lines lines using different concentrations. The extract and standard chemotherapy agent (cisplatin) were used in time dependent manner to investigate the IC50. To compare the cytotoxicities, cells without being exposed to extracts were used as control. Cells were incubated with extracts and cytotoxicity was determined by the MTT assay. The experiments were repeated 3 times and IC50 values were calculated. Data collected using the software Graph Pad Prism-6, IC50 extract obtained in three different time periods. Results: According to the results, it can be seen that the plant extract does not have a significant effect on MCF-7 cancer cells in all three time intervals of 24, 48 and 72 hours. The IC50 of anti-cancer drug cisplatin in 48 and 72 hours, are respectively 22 μg / mL (73 μmol) and 4.92 μg / mL (16 μmol). Also, with increasing time, the effect of cisplatin on healthy cells of HuGu is increased, but the plant extract does not affect the healthy cells of HuGu. Conclusion: In general, plant extract, in these concentrations, unlike anticancer cisplatin, has no inhibitory effect on MCF-7 and healthy cells of HuGu.
The Antimicrobial activities of Magnesium oxide nanoparticles on soft contact lenses
(Tabriz University of Medical Science, School of pharmacy, 2021) asadi, Bahareh; Adibkia, Khosro; Mohajeri, Seyed ahmad; Khameneh, Bahman
Increasing resistance of pathogens to antibiotics in recent years has created many problems in the medical community. The combination of modern technology such as nanotechnology with metallic elements with antimicrobial properties has been able to create a new path in the fight against resistant microorganisms. Magnesium oxide nanoparticles are a metal oxide that is widely used in various fields. These metal nanoparticles have shown good bactericidal performance in different environments due to the production of superoxide anions. For this reason, these particles can be used alone or in combination with antibiotics to prevent bacterial activity. AIM: Evaluation of antimicrobial properties of magnesium oxide nanoparticles in the presence of contact lenses to prevent contamination of lenses in non-sterile medium and to determine the potential ability of magnesium oxide to inhibit microbial growth. Methods: Firstly,The MIC (Minimum inhibitory concentration) and MBC (Minimum Bactericidal Concentration) of magnesium oxide nanoparticles for Candida albicans, Staphylococcus aurous and epidermidis, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis was determined. Then the inactivation kinetics test and anti-biofilm effects of nanoparticles on contact lenses were evaluared by plate microtiter,microbial counting and microscopic electron imaging (by 3 methods) Results: Magnesium oxide nanoparticles showed a significant effect in reducing the number of bacteria in 24 hours after culture time and caused a reduction of 2-3 logarithms in the number of bacteria. These particles have anti-biofilm properties, but these effects are not statistically significant. Conclusions: Therefore, it can be concluded that magnesium oxide nanoparticles can be used as a preservative, antibiotic in the formulation of contact lenses.
Application of aerogel for extraction and preconcentration of risperidone in plasma samples and the determination with liquid chromatography
(Tabriz University of Medical Sciences , School of Pharmacy, 2022) Feyzi, Faez; Ranjbar, Fatemeh; Jouyban, Abolghasem; Soleymani, Jafar
Risperidone (RISP) is belonging to the chemical class of benzisoxazole derivatives and chemically, it is 4-[2-[4-(6- fluorobenzo[d]isoxazol-3-yl)-1-piperidyl] ethyl]-3- methyl-2, 6 diazabicyclo [4.4.0] deca-1, 3-dien-5-one with molecular formula C23H27FN4O2 and CAS number 106266-06-2. In children and adolescents, risperidone is used for a range of disorders, such as behavioural problems associated with developmental disorders, disruptive behaviour disorders and tic disorders in addition to psychotic disorders.Target:In the present article we describe an innovative simple, rapid and sensitive HPLC method for the simultaneous determination of risperidone in plasma using a simple Solid– liquid extraction. The assay involves a short chromatographic run and fulfils the requirements for use in therapeutic drug monitoring.Method:A graphene oxide (GO)-based aerogel was synthesized and applied to the extraction and the determinations with the HPLC-UV. After the characterization of the produced GO-aerogel, it was utilized as a dispersive solid-phase extraction (DSPE) sorbent for risperidone (RIS) extraction from plasma samples. Aerogels are materials with a surface area-to-mass ratio and plenty of core with functional groups which can easily attach to the analytes to extract them to the second phase.Results:The suggested method determined RISP in plasma samples in the wide dynamic range from 20 ng/mL to 3 µg/mL. The LOD and LOQ of the developed method were calculated as 2.4 ng/mL and 8 ng/mL, respectively. This method has no need to precipitate plasma proteins, improving the analytical performance of the analysis. The obtained results proposed that the developed approach could be employed as an accurate method for quantification of RIS in the real plasma samples.Discuss:The designed method has the ability to determine the concentration of Risperidone in plasma and can be used to analyze plasma samples containing Risperidone.
Application of capillary electrophoresis in stability studies of some biopharmaceutical products.
(Tabriz University of Medical Sciences, Faculty of Pharmacy, 1393) Tamizi, Elnaz; Jouyban, Abolghasem
Since inherent physicochemical complexity of biopharmaceuticals makes them susceptible to the chemical and physical instability, one of the crucial parts of their quality control is stability studies. It is obvious that, without reliable analytical tools with a stability indicating capacity, stability studies are not possible. Capillary electrophoresis (CE) is a poweful analytical technique and along with the extreme advancements in biopharmaceutical industry over the past two decades, considerable effort has been dedicated toward developing CE-based methods applicable for the characterization and quality control of biopharmaceuticals. Considering the importance of stability studies in the quality control of biopharmaceuticals and the important role of capillary electro-migration methods in their quality control, the main objective of this thesis is application of CE in the stability studies of some biopharmaceutical products. To investigate the usefulness of CE technique in the stability studies of biopharmaceuticals, heparin as a complex carbohydrate, buserelin as a linear peptide and octreotide as a cyclic peptide have been selected as candidate biopharmaceuticals. Then, some novel stability indicating CE-based methods have been developed and validated for the quantitative analysis of candidate biopharmaceutical products, in both the pharmaceutical formulations and force degraded samples. Afterward, the developed methods have been successfully employed for the evaluation of the degradation behaviors of examined biopharmaceuticals under stress conditions and estimation of the involved kinetics in their degradation. Moreover, the application of CE in combination with ion trap mass spectrometry (IT-MS) has been explored for the reliable analysis of octreotide, identification of its major degaradtion products and investigation of the degradation pathways responsible for their formation under stress conditions. In addition, a review about the applications of electro-migration methods in the quality control of biopharmaceuticals and a summarized review about the stability studies of biopharmaceuticals under stress conditions have been prepared. By taking the findings of the present thesis into the consideration, it can be concluded that, CE-based methods could be successfully applied as reliable tools in quality control laboratories to assess the quality and stability of biopharmaceuticals in bulk and finished products. In addition CE-based methods particularly in combination with mass detector could play an important role in the preparation of the stability profiles of biopharmaceuticals through the investigation of the kinetics could govern their degradation, characterization of the pathways could involve in their degradation and identification of their possible degradation products.
Application of coupled ionic liquid to silica nanoparticle as adsorbent in mixed hemimicelle dispersive solid phase extraction of methamphetamine from urine
(Tabriz University of Medical Science, School of pharmacy, 2022) boyaghchi, Sepideh; Taghvimi, Arezou; Javadzadeh, Yousef; Dastmalchi, Siavoush
Methamphetamine misusage is a great concern worldwide. The growing trend of methamphetamine abuse leads to crime among young people. Methamphetamine stimulates the central nervous system and makes mental and physical disorders. Therefore, methamphetamine determination is of great interest to researchers in forensic laboratories. Aim: coupled ionic liquid to silica nanoparticles was synthesized and applied as a new adsorbent in dispersive solid-phase extraction (DSPE) of methamphetamine from human urine samples. Material and method : The Stober method was applied for the synthesis of nano-silica. Preparation of coupled ionic liquid to silica nanoparticles was achieved. After full characterization of the prepared adsorbent by various techniques, the nanocomposite was used in dispersive solid-phase extraction of methamphetamine. Effective extraction parameters were optimized by one- parameter-at-a-time method. Analysis of real samples was carried out by the proposed method. Results: coupled ionic liquid to silica nanoparticles was used for efficient extraction of methamphetamine. Silica nano adsorbents were characterized by different techniques such as IR, XRD, SEM, and zeta potential, and then optimization of effective parameters in the extraction process was performed. The synthesis method of silica nano adsorbent was easy, and reproducible responses were obtained in methamphetamine extraction. The calibration curve was plotted in the concentration range of 50-2000 ng/mL and validation of the method was carried out. limit of detection was 9 ng/mL.Discussion: The analysis of methamphetamine in the urine samples from addicted subjects was carried out using the prepared nanocomposite-based method followed by HPLC. Determination with the proposed method confirmed the applicability of the method in different laboratories.
Application of Graphene Quantom dot on determination of ِDoxorubicin on biological samples
(Tabriz University of Medical Science, School of pharmacy, 2015) Hashemzadeh, Nastaran; Jouyban, Abolghasem; Shadjou, Nasrin
Introduction: Nowadays anticancer drugs which used as chemotherapy regimen for patients are become more useful. The development of an accurate and repeatable method for determination of these drugs, will improve our understanding of their role in the treatment process. One of the common methods of determining the trace amount of these drugs is electrochemical methods. According to the importance of these methods in the analysis of drugs, in the present study graphene quantum dots (GQDs) used as active ingredient in the preparation of electrochemical sensors. Application of prepared electrochemical sensor for the detection and quantification of doxorubicin in biological samples were studied. Aim: Developing an electrochemical sensor based on graphene quantum dots and its application for the detection and determination of doxorubicin (DOX) in biological samples. Methods: In this study, GQDs were synthesized by pyrolyzing citric acid in alkaline solution.The UV–Vis spectroscopy, X-ray diffraction (XRD), transition electron microscopy (TEM), Fourier transform infrared (Ft-IR) spectroscopy, Atomic force microscopy (AFM) and cyclic and differential pulse voltammetric techniques were used for characterizing synthesized GQDs. The electrochemical behavior of GQD modified glassy carbon electrode (GQD-GCE) was studied using cyclic voltammetry (CV) technique. The electrochemical behavior of DOX was investigated at the GQD-GCE in phosphate buffer solution (PBS), using differential pulse voltammetry technique (DPV). Results and Conclusion: electrochemical behavior GQD-GCE was studied using CV and DPV. DPV was used to evaluate the analytical performance of DOX in the presence of PBS (pH 4) and good limit of detection was obtained by proposed sensor. The results revealed that GQD promotes the rate of oxidation by increasing the peak current. Finally, the applicability of the proposed method was described to the direct assays of spiked human plasma.