School of Medicine

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The Faculty of Medicine is one of the premier medical research and educational institutions in Iran for over 50 years.As a fist type institution in Iran, the faculty provides best educational and research programs in the areas of health and life sciences through an expert staff and province-wide model. Our faculty members, students, staff and alumni are actively engaged in leading edge research, education and community service on university and hospital campuses across the province....

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Author: search.filters.author.Alipour, Maedeh

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    Chemotherapy Effects on PD-L1, CTLA-4, and VISTA Gene Expression in a Prostate Cancer Cell Line
    (Tabriz University of Medical Sciences, Faculty of Medicine, 2024) Alipour, Maedeh; Kazemi, Tohid; Agebati, Leyli; Baradaran, Behzad; Ahmadian, Javad
    Globally, prostate cancer was the second most common cancer and the fifth cause of cancer-related deaths among men. Metastatic prostate cancer is a fatal disease with limited treatment options. Immune checkpoint inhibitors have dramatically changed the treatment landscape for all types of cancers. Conventional chemotherapies have become useful clinical agents based on their ability to preferentially kill malignant cells. Nevertheless, the clinical activity of various chemotherapies is now known to be due to the stimulation of anticancer immunity by initiating the release of immune stimulatory molecules from dying cancer cells or by mediating the immune cell population. Therefore, determining the effects of chemotherapy The treatment of the taxane family is important on the immune system, especially on the expression of checkpoint receptors. Materials and methods: For this purpose, prostate cancer cells were cultured in RPMI culture medium containing 10% FBS at 37ₒc and 5% CO2, then the expression level of CTLA-4, PD-L1, VISTA gene in different cancer types was determined. It was measured by qRT-PCR. After adding common chemotherapy drugs including doxorubicin (paclitaxel) and docetaxel (docetaxel), the rate of growth and proliferation of cancer cells was examined by the MTT technique, and for this, the cells were exposed to the drugs and then after 24 hours, the environment The culture was replaced with culture medium containing MTT and after 4 hours of incubation, the light absorption was read by ELISA Plate Reader at 570 nm wavelength. Also, the expression level of CTLA-4, PD-L1, VISTA gene in different prostate cancer types after adding chemotherapy drugs was measured by qRT-PCR. Results: The results of immunocheckpoint gene expression showed that the expression level of CTLA-4, PD-L1, and VISTA genes increased, while the expression level of the genes decreased significantly by treating the cell line with chemotherapy drugs.
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    Effects of transcranial near-infrared photobiomodulation on electrical activity of hippocampal neurons in a rat model of Alzheimer's disease
    (Tabriz University of Medical Sciences, Faculty of Medicine, 2024) Alipour, Maedeh; Mahmoudi, Javad; Sadigh-Eteghad, Saeed; Farajdokht, Fereshteh
    Alzheimer's disease is one of the most common causes of dementia in old age, so that about 50 million people around the world are suffering from dementia and 50-70% of them are suffering from Alzheimer's disease. Considering that the commonly used drugs are not effective due to side effects and various drug interactions, it seems necessary to use new treatment methods for the treatment of Alzheimer's disease. The present study was conducted to investigate the effect of transcranial near-infrared laser (PMB) radiation on changes in the electrical activity of single neurons in the hippocampus of Alzheimer's model rats to find a new treatment method. Materials and methods: 48 adult male Wistar rats (weighing 250-300 g) were used in this study. The animals were randomly divided into 6 groups of eight including control, surgical sham, Alzheimer's model (AD), AD+ treatment with PBM 8 J, AD+ treatment with PBM 16 J, and AD+ treatment with PBM 32 J. Alzheimer's model was induced by injecting amyloid beta (Aβ1-42) into the lateral ventricles of the brain. In the treatment groups, rats were exposed to 810 nm PBM with energies of 8, 16, and 32 joules daily for 14 consecutive days. Two weeks after the surgery, the T-Maze test was performed to check the animal's memory. At the end, the electrical activity of the hippocampal neurons was evaluated by the electrophysiology protocol, and the rate of nerve firing and the frequency of spike activities were recorded in different groups. Results: 8 J laser irradiation for 14 days in AD rats significantly improved the memory of AD animals in the T-Maze test compared to the AD group. Also, the results of single-unit electrophysiological recording showed that 8 J laser increases the rate of nerve firing and the frequency of spike activities in AD animals.