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Subject: search.filters.subject.Human plasma

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    Application of aerogel for extraction and preconcentration of risperidone in plasma samples and the determination with liquid chromatography
    (Tabriz University of Medical Sciences , School of Pharmacy, 2022) Feyzi, Faez; Ranjbar, Fatemeh; Jouyban, Abolghasem; Soleymani, Jafar
    Risperidone (RISP) is belonging to the chemical class of benzisoxazole derivatives and chemically, it is 4-[2-[4-(6- fluorobenzo[d]isoxazol-3-yl)-1-piperidyl] ethyl]-3- methyl-2, 6 diazabicyclo [4.4.0] deca-1, 3-dien-5-one with molecular formula C23H27FN4O2 and CAS number 106266-06-2. In children and adolescents, risperidone is used for a range of disorders, such as behavioural problems associated with developmental disorders, disruptive behaviour disorders and tic disorders in addition to psychotic disorders.Target:In the present article we describe an innovative simple, rapid and sensitive HPLC method for the simultaneous determination of risperidone in plasma using a simple Solid– liquid extraction. The assay involves a short chromatographic run and fulfils the requirements for use in therapeutic drug monitoring.Method:A graphene oxide (GO)-based aerogel was synthesized and applied to the extraction and the determinations with the HPLC-UV. After the characterization of the produced GO-aerogel, it was utilized as a dispersive solid-phase extraction (DSPE) sorbent for risperidone (RIS) extraction from plasma samples. Aerogels are materials with a surface area-to-mass ratio and plenty of core with functional groups which can easily attach to the analytes to extract them to the second phase.Results:The suggested method determined RISP in plasma samples in the wide dynamic range from 20 ng/mL to 3 µg/mL. The LOD and LOQ of the developed method were calculated as 2.4 ng/mL and 8 ng/mL, respectively. This method has no need to precipitate plasma proteins, improving the analytical performance of the analysis. The obtained results proposed that the developed approach could be employed as an accurate method for quantification of RIS in the real plasma samples.Discuss:The designed method has the ability to determine the concentration of Risperidone in plasma and can be used to analyze plasma samples containing Risperidone.
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    Application of Graphene Quantom dot on determination of ِDoxorubicin on biological samples
    (Tabriz University of Medical Science, School of pharmacy, 2015) Hashemzadeh, Nastaran; Jouyban, Abolghasem; Shadjou, Nasrin
    Introduction: Nowadays anticancer drugs which used as chemotherapy regimen for patients are become more useful. The development of an accurate and repeatable method for determination of these drugs, will improve our understanding of their role in the treatment process. One of the common methods of determining the trace amount of these drugs is electrochemical methods. According to the importance of these methods in the analysis of drugs, in the present study graphene quantum dots (GQDs) used as active ingredient in the preparation of electrochemical sensors. Application of prepared electrochemical sensor for the detection and quantification of doxorubicin in biological samples were studied. Aim: Developing an electrochemical sensor based on graphene quantum dots and its application for the detection and determination of doxorubicin (DOX) in biological samples. Methods: In this study, GQDs were synthesized by pyrolyzing citric acid in alkaline solution.The UV–Vis spectroscopy, X-ray diffraction (XRD), transition electron microscopy (TEM), Fourier transform infrared (Ft-IR) spectroscopy, Atomic force microscopy (AFM) and cyclic and differential pulse voltammetric techniques were used for characterizing synthesized GQDs. The electrochemical behavior of GQD modified glassy carbon electrode (GQD-GCE) was studied using cyclic voltammetry (CV) technique. The electrochemical behavior of DOX was investigated at the GQD-GCE in phosphate buffer solution (PBS), using differential pulse voltammetry technique (DPV). Results and Conclusion: electrochemical behavior GQD-GCE was studied using CV and DPV. DPV was used to evaluate the analytical performance of DOX in the presence of PBS (pH 4) and good limit of detection was obtained by proposed sensor. The results revealed that GQD promotes the rate of oxidation by increasing the peak current. Finally, the applicability of the proposed method was described to the direct assays of spiked human plasma.
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    Application of Graphene Quantom dot on determination of ِDoxorubicin on biological samples
    (Tabriz University of Medical Sciences, School of Pharmacy, 2015) Hashemzadeh, Nastaran; Jouyban, Abolghasem; Shadjou, Nasrin
    Introduction: Nowadays anticancer drugs which used as chemotherapy regimen for patients are become more useful. The development of an accurate and repeatable method for determination of these drugs, will improve our understanding of their role in the treatment process. One of the common methods of determining the trace amount of these drugs is electrochemical methods. According to the importance of these methods in the analysis of drugs, in the present study graphene quantum dots (GQDs) used as active ingredient in the preparation of electrochemical sensors. Application of prepared electrochemical sensor for the detection and quantification of doxorubicin in biological samples were studied. Aim: Developing an electrochemical sensor based on graphene quantum dots and its application for the detection and determination of doxorubicin (DOX) in biological samples. Methods: In this study, GQDs were synthesized by pyrolyzing citric acid in alkaline solution.The UV–Vis spectroscopy, X-ray diffraction (XRD), transition electron microscopy (TEM), Fourier transform infrared (Ft-IR) spectroscopy, Atomic force microscopy (AFM) and cyclic and differential pulse voltammetric techniques were used for characterizing synthesized GQDs. The electrochemical behavior of GQD modified glassy carbon electrode (GQD-GCE) was studied using cyclic voltammetry (CV) technique. The electrochemical behavior of DOX was investigated at the GQD-GCE in phosphate buffer solution (PBS), using differential pulse voltammetry technique (DPV). Results and Conclusion: electrochemical behavior GQD-GCE was studied using CV and DPV. DPV was used to evaluate the analytical performance of DOX in the presence of PBS (pH 4) and good limit of detection was obtained by proposed sensor. The results revealed that GQD promotes the rate of oxidation by increasing the peak current. Finally, the applicability of the proposed method was described to the direct assays of spiked human plasma.