Theses(P)

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Author: search.filters.author.Hashemzadeh, NastaranHas files: Yes

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    Application of Graphene Quantom dot on determination of ِDoxorubicin on biological samples
    (Tabriz University of Medical Science, School of pharmacy, 2015) Hashemzadeh, Nastaran; Jouyban, Abolghasem; Shadjou, Nasrin
    Introduction: Nowadays anticancer drugs which used as chemotherapy regimen for patients are become more useful. The development of an accurate and repeatable method for determination of these drugs, will improve our understanding of their role in the treatment process. One of the common methods of determining the trace amount of these drugs is electrochemical methods. According to the importance of these methods in the analysis of drugs, in the present study graphene quantum dots (GQDs) used as active ingredient in the preparation of electrochemical sensors. Application of prepared electrochemical sensor for the detection and quantification of doxorubicin in biological samples were studied. Aim: Developing an electrochemical sensor based on graphene quantum dots and its application for the detection and determination of doxorubicin (DOX) in biological samples. Methods: In this study, GQDs were synthesized by pyrolyzing citric acid in alkaline solution.The UV–Vis spectroscopy, X-ray diffraction (XRD), transition electron microscopy (TEM), Fourier transform infrared (Ft-IR) spectroscopy, Atomic force microscopy (AFM) and cyclic and differential pulse voltammetric techniques were used for characterizing synthesized GQDs. The electrochemical behavior of GQD modified glassy carbon electrode (GQD-GCE) was studied using cyclic voltammetry (CV) technique. The electrochemical behavior of DOX was investigated at the GQD-GCE in phosphate buffer solution (PBS), using differential pulse voltammetry technique (DPV). Results and Conclusion: electrochemical behavior GQD-GCE was studied using CV and DPV. DPV was used to evaluate the analytical performance of DOX in the presence of PBS (pH 4) and good limit of detection was obtained by proposed sensor. The results revealed that GQD promotes the rate of oxidation by increasing the peak current. Finally, the applicability of the proposed method was described to the direct assays of spiked human plasma.
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    Targeted therapy of breast cancer using methotrexate and indolamine-2,3-dioxygenase inhibitor loaded mesoporous silica magnetic nanoparticles
    (Tabriz University of Medical Sciences, Faculty of Pharmacy, 2021) Hashemzadeh, Nastaran; Omidi, Yadollah; Barzegar Jalali, Mohammad; Aghanezhad, Ayoub; Barar, Jaleh; Adibkia, Khosro
    Introduction: Conventional cancer therapeutic agents often are associated with the serious challenge due to adverse effects. Whereases, the emergence of targeted drug delivery vehicles provides an alternative approach for cancer therapy. Recently, cancer immunotherapy agents such as 1-Methyl-D Tryptophan (1-MDT) have been mostly used in cancer therapy. Aim: We established the PEGylated targeted drug delivery system using mesoporous silica magnetic nanoparticles (MSMNPs) for encapsulating 1-Methyl-D Tryptophan (1-MDT) as an immunotherapeutic and investigated its anti-tumor efficiency in combination with methotrexate (MTX) on breast cancer cell lines. Methods: In the present study, the thermal decomposition technique was used to synthesize superparamagnetic iron oxide nanoparticles (SPIONs). In the next step, these SPIONs were coated with mesoporous silica and conjugated with Polyethylene glycol (PEG) 2100 polymer and methotrexate in various steps to achieve MTX-PEG-MSMNPs. Subsequently, 1-MDT was loaded onto the final particles. Prepared NPs were characterized beyond each synthetic step using TEM, SEM, DLS, ATR-FTIR, VSM, BET, and XRD. Thereupon, quantitative RT- PCR was used to investigate the effect of 1-MDT on the expression of indoleamine 2,3 dioxygenase (IDO) on cancer cells. Besides, flow cytometry was performed to evaluate the cellular uptake. Subsequently, the antiproliferative effect of NPs was explored using MTT, NIR, and apoptosis assays in cancer cells. Results: The results revealed that the MTX-PEG-MSMNPs had a uniform size distribution (average size = 68.33, PDI=0.16). The Encapsulation efficiency and Loading capacity respectively were about 71% and 60%. Uptake experiments demonstrated that prepared nanoparticles can target breast cancer cells. RT-PCR data indicated that 1-MDT suppressed the IDO level. Besides, cytotoxic and apoptosis assays further confirmed the efficiency of NPs in cancer cell growth inhibition. Moreover, in-vitro MTT analysis revealed that 1-MDT loaded in MTX-PEG-MSMNPs in combination with NIR declined the cell viability. Conclusion: Overall, the engineered PEGylated MTX- armed mesoporous silica magnetic nanoparticles had potential anti-tumor impacts in vitro.
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    Application of Graphene Quantom dot on determination of ِDoxorubicin on biological samples
    (Tabriz University of Medical Sciences, School of Pharmacy, 2015) Hashemzadeh, Nastaran; Jouyban, Abolghasem; Shadjou, Nasrin
    Introduction: Nowadays anticancer drugs which used as chemotherapy regimen for patients are become more useful. The development of an accurate and repeatable method for determination of these drugs, will improve our understanding of their role in the treatment process. One of the common methods of determining the trace amount of these drugs is electrochemical methods. According to the importance of these methods in the analysis of drugs, in the present study graphene quantum dots (GQDs) used as active ingredient in the preparation of electrochemical sensors. Application of prepared electrochemical sensor for the detection and quantification of doxorubicin in biological samples were studied. Aim: Developing an electrochemical sensor based on graphene quantum dots and its application for the detection and determination of doxorubicin (DOX) in biological samples. Methods: In this study, GQDs were synthesized by pyrolyzing citric acid in alkaline solution.The UV–Vis spectroscopy, X-ray diffraction (XRD), transition electron microscopy (TEM), Fourier transform infrared (Ft-IR) spectroscopy, Atomic force microscopy (AFM) and cyclic and differential pulse voltammetric techniques were used for characterizing synthesized GQDs. The electrochemical behavior of GQD modified glassy carbon electrode (GQD-GCE) was studied using cyclic voltammetry (CV) technique. The electrochemical behavior of DOX was investigated at the GQD-GCE in phosphate buffer solution (PBS), using differential pulse voltammetry technique (DPV). Results and Conclusion: electrochemical behavior GQD-GCE was studied using CV and DPV. DPV was used to evaluate the analytical performance of DOX in the presence of PBS (pH 4) and good limit of detection was obtained by proposed sensor. The results revealed that GQD promotes the rate of oxidation by increasing the peak current. Finally, the applicability of the proposed method was described to the direct assays of spiked human plasma.