Investigation of P16INK4A and P14ARF gene expression changes in diffuse nasal polyp tissue and comparing it with surrounding peripheral tissue

Abstract

Sinonasal malignancies are one of the causes of death. Every year, 1000 new cases of this waste are discovered and diagnosed in the United States of America, and it is the cause of 300 deaths in the United States. Inverted papilloma has been associated with human papilloma virus in many cases. The INK4A/ARF regulatory pathway is disrupted by human papillomavirus infection and is therefore a suitable candidate to study in the pathogenesis of inverted papilloma, which has two proteins, P16INK4A and P14ARF. P16INK4A and P14ARF play a vital role in maintaining cellular homeostasis and preventing the oncogenic process. Methods: In this research, 40 tissue samples of people suffering from this disease were examined. Cytoplasmic RNA was extracted from the desired tissue using Trizol, cDNA was synthesized by a special kit and used for gene analysis by Real Time PCR technique, finally statistical analysis was performed to compare and compare between groups. Results: In this study, the results showed a comparison of the expression of P16INK4A and P14ARF genes in the polyp tissue and the surrounding margin in the form of an increase in the expression of P16INK4A (p=0.0033) and a decrease in the expression of P14ARF (p=0.0089) in the tumor. Also, the results of the ROC Curve in P14ARF (p=0.11), (Area:0.59) and in P16INK4A gene ((p=0.0088), (Area:0.63) were obtained. Conclusion: