Assessment of follicular development, antioxidant capacity and revascularization of grafted ovarian tissue in encapsulated with sodium selenite

Abstract

One of the ways to preserve fertility in women with cancer is ovarian tissue transplantation. Measures such as the use of scaffolds, antioxidants, growth factors and hormones have been used to accelerate the resumption of ovarian activities and the survival of follicles in the transplanted ovarian tissue. The aim of this study was to investigate follicular growth, antioxidant capacity and vascularization of ovarian graft enclosed in fibrin scaffold with sodium selenite in order to increase the rate of angiogenesis and reduce oxidative stress in transplanted ovarian tissue. Materials and Methods: In this experimental study, 20 adult female rats (8 weeks) underwent ovariectomy. Then their ovaries were divided into four groups: control, control /transplantation (T), T with fibrin scaffold (T+H), T+H with sodium selenite (T+H+SS). Finally, the ovaries autotransplantation in the rats' lumbar region. After 14 days, the ovaries were removed. The level of antioxidants (SOD, GPx, MDA and TAC) was evaluated by the ELISA technique, and expressions of CD34 and VEGF and ANGPT2 were related to the apoptosis by real-time RT-PCR. Also, blood serum samples were extracted to evaluate FSH, LH and E2 hormones and for evaluating the follicle quality H&E staining was done. Result: The results of the present study showed that the level of antioxidant enzymes and total antioxidant capacity were not significantly different in the treatment groups, and the level of malondialdehyde increased in the transplanted ovary group and decreased in the antioxidant receiving groups. The number of follicles in the transplanted ovary group decreased compared to the control and increased in the treatment group.