The effect of bee pollen crude extract and pentoxifylline on the prevention of vincristine-induced neuropathy in mice
چکیده
Introduction: Anticancer drugs can induce neuropathy through various mechanisms, including neuronal inflammation, oxidative stress, and heightened activity of NMDA and serotonin receptors. Bee pollen is known for its anti-inflammatory and antioxidant properties, while pentoxifylline, a blood-thinning medication, has been shown to decrease inflammatory parameters.Objective: The aim of this study was to evaluate the effects of bee pollen and pentoxifylline in preventing vincristine-induced neuropathy in mice.Materials and methods: Male mice (N=81; weight: 25-35 g)were divided into 9 groups. Different doses of bee pollen (100, 200, 400 mg/kg, ip) and pentoxifylline (25, 50, 100 mg/kg, ip) or saline were injected three days before vincristine injection, and on the fourth day, vincristine (1 mg/kg, ip) was administered as a single dose. Vincristine was then injected with drugs for 10 days until the 14th day Vincristine was discontinued from the 14th day until the 17th day, and only drugs were injected. At the end of the 17th day, blood was collected from the hearts of the animals for MDA and TAC tests.Results: The results indicated that the doses of bee pollen and pentoxifylline produced significant changes in vincristine-induced neuropathy. The doses of pentoxifylline and bee pollen produced significant changes in serum MDA and TAC levels.Conclusion: Considering the concordance of the results of the behavioral study (neuropathy prevention) with changes in MDA and TAC levels, it is concluded that part of the mechanism of benefit of different doses of pentoxifylline and bee pollen in preventing vincristine-induced neuropathy is by inhibiting the oxidative stress system. Additional studies are recommended to evaluate the possible effect of bee pollen and pentoxifylline in preventing neuropathy in humans.