Intradermal lymphocyte therapy in recurrent pregnancy loss (RPL) patients with anti-TPO antibodies and patients without Anti-TPO

Abstract

In recent studies, the discussion and exchange of opinions in the field of immunotherapy for reproductive patients, especially those with recurrent miscarriages (RPL), is very interesting. Limited studies are dedicated to investigating the effect of intradermal lymphocyte therapy in improving the pregnancy rate in women with RM. Autoimmune thyroid diseases can be easily determined by measuring anti-TPO and TG antibodies in the blood circulation. About 5-15% of euthyroid women and 2% of euthyroid men have anti-thyroid antibodies. Such people are at high risk of developing thyroid dysfunction. If the Anti-TPO test is strongly positive, it indicates an autoimmune thyroid disease. Therefore, in this study, we examined the immunological factors in two separate groups of patients with and without TPO antibodies. Materials and methods: This study was done in a descriptive-analytical way. After taking heparinized blood samples from the patient group and the control group (50 people in each group), the peripheral blood mononuclear cells of PBMC were separated using Ficoll and density gradient centrifugation. To check the frequency of Treg, Th17, Th1, and Th2 cells in the peripheral blood, flow cytometry was used, Realtime-PCR was used to check the gene expression of cytokines and transcription factors, and ELISA was used to check the secretion of cytokines. Then, data distribution was checked using prism software and Test-T test was used for normal distribution and Whitney-Mann test was used for data with non-normal distribution. Results: The results of the study showed that the level of expression and secretion of Th1 and Th17 inflammatory cytokines is generally higher in patients than normal people, and this level of expression is different between the two groups of patients, each of which is divided according to the positive and negative anti-TPO. Also, the level of expression and secretion of Th2 and regulatory T factors in patients is lower than that of healthy people, and also, in general, this level of expression is different between two groups of patients, each of which is divided according to positive and negative anti-TPO.